小柯机器人

科学家绘制人类巨噬细胞发育图谱
2020-05-22 13:37
来源：小柯机器人

中国人民解放军总医院第五医学中心刘兵、新加坡免疫协作组Florent Ginhoux、暨南大学基础医学院兰雨等研究人员合作绘制了人类巨噬细胞的发育图谱。2020年5月20日，《自然》在线发表了这一成果。

研究人员从卡内基阶段第11至23期的人类胚胎中分离了CD45+造血细胞，并通过单细胞RNA测序对它们进行了转录组分析，然后对CD45+CD34+CD44+卵黄囊来源的髓样倾向祖细胞（YSMP）进行功能表征。
 
研究人员还绘制了多个解剖部位的巨噬细胞异质性，并鉴定了不同的亚群，包括各种类型的胚胎组织驻留巨噬细胞（TRM）（在头部、肝脏、肺和皮肤中）。研究人员进一步使用转录组学和发育分期信息，从卵黄囊来源的原始巨噬细胞或YSMP来源的胚胎肝单核细胞追踪了TRM的分化轨迹。
 
最后，研究人员评估了胚胎TRM与成年TRM之间的分子相似性。这些数据是人类胚胎发生过程中早期巨噬细胞发育的时空动态全面表征，为人类TRM的发育和功能研究提供了参考。
 
据悉，巨噬细胞是在胚胎发育过程中新生免疫系统的第一批细胞。在小鼠中，胚胎巨噬细胞浸入发育中的器官，在那里它们共生地分化为组织驻留巨噬细胞（TRM）。但是，我们对人类胚胎中巨噬细胞起源和分化的了解是有限的。
 
附：英文原文

Title: Deciphering human macrophage development at single-cell resolution

Author: Zhilei Bian, Yandong Gong, Tao Huang, Christopher Z. W. Lee, Lihong Bian, Zhijie Bai, Hui Shi, Yang Zeng, Chen Liu, Jian He, Jie Zhou, Xianlong Li, Zongcheng Li, Yanli Ni, Chunyu Ma, Lei Cui, Rui Zhang, Jerry K. Y. Chan, Lai Guan Ng, Yu Lan, Florent Ginhoux, Bing Liu

Issue&Volume: 2020-05-20

Abstract: Macrophages are the first cells of the nascent immune system to emerge during embryonic development. In mice, embryonic macrophages infiltrate developing organs, where they differentiate symbiotically into tissue-resident macrophages (TRMs)1. However, our understanding of the origins and specialization of macrophages in human embryos is limited. Here we isolated CD45+ haematopoietic cells from human embryos at Carnegie stages 11 to 23 and subjected them to transcriptomic profiling by single-cell RNA sequencing, followed by functional characterization of a population of CD45+CD34+CD44+ yolk sac-derived myeloid-biased progenitors (YSMPs) by single-cell culture. We also mapped macrophage heterogeneity across multiple anatomical sites and identified diverse subsets, including various types of embryonic TRM (in the head, liver, lung and skin). We further traced the specification trajectories of TRMs from either yolk sac-derived primitive macrophages or YSMP-derived embryonic liver monocytes using both transcriptomic and developmental staging information, with a focus on microglia. Finally, we evaluated the molecular similarities between embryonic TRMs and their adult counterparts. Our data represent a comprehensive characterization of the spatiotemporal dynamics of early macrophage development during human embryogenesis, providing a reference for future studies of the development and function of human TRMs.

DOI: 10.1038/s41586-020-2316-7

Source: https://www.nature.com/articles/s41586-020-2316-7

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
官方网址：http://www.nature.com/
投稿链接：http://www.nature.com/authors/submit_manuscript.html


本期文章：《自然》：Online/在线发表

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