广州医科大学第一附属医院赵金存、美国爱荷华大学Stanley Perlman、Paul B. McCray Jr.等研究人员合作开发了新冠感染非转基因小鼠模型。这一研究成果于2020年6月10日在线发表在《细胞》上。
Title: Generation of a Broadly Useful Model for COVID-19 Pathogenesis Vaccination, and Treatment
Author: Jing Sun, Zhen Zhuang, Jian Zheng, Kun Li, Roy Lok-Yin Wong, Donglan Liu, Jicheng Huang, Jiangping He, Airu Zhu, Jingxian Zhao, Xiaobo Li, Yin Xi, Rongchang Chen, Abeer N. Alshukairi, Zhao Chen, Zhaoyong Zhang, Chunke Chen, Xiaofang Huang, Fang Li, Xiaomin Lai, Dingbin Chen, Liyan Wen, Jianfen Zhuo, Yanjun Zhang, Yanqun Wang, Shuxiang Huang, Jun Dai, Yongxia Shi, Kui Zheng, Mariah R. Leidinger, Jiekai Chen, Yimin Li, Nanshan Zhong, David K. Meyerholz, Paul B. McCray, Stanley Perlman, Jincun Zhao
Issue&Volume: 2020-06-10
Abstract: COVID-19, caused by SARS-CoV-2, is a virulent pneumonia, with >4,000,000 confirmed cases worldwide and >290,000 deaths as of May 15, 2020. It is critical that vaccines and therapeutics be developed very rapidly. Mice, the ideal animal for assessing such interventions, are resistant to SARS-CoV-2. Here, we overcome this difficulty by exogenous delivery of human ACE2 with a replication-deficient adenovirus (Ad5-hACE2). Ad5-hACE2-sensitized mice developed pneumonia characterized by weight loss, severe pulmonary pathology, and high-titer virus replication in lungs. Type I interferon, T cells and, most importantly, signal transducer and activator of transcription 1 (STAT1) are critical for virus clearance and disease resolution in these mice. Ad5-hACE2-transduced mice enabled rapid assessments of a vaccine candidate, of human convalescent plasma, and of two antiviral therapies (poly I:C and remdesivir). In summary, we describe a murine model of broad and immediate utility to investigate COVID-19 pathogenesis, and to evaluate new therapies and vaccines.
DOI: 10.1016/j.cell.2020.06.010
Source: https://www.cell.com/cell/fulltext/S0092-8674(20)30741-8
本期文章:《细胞》:Online/在线发表