小柯机器人

美国洛杉矶儿童医院Chintan Parekh研究小组利用人类胸腺单细胞RNA测序，揭示出T细胞发育过程中的分化轨迹。该研究于2020年6月16日发表于《免疫》。

Title: Single-Cell RNA-Seq Mapping of Human Thymopoiesis Reveals Lineage Specification Trajectories and a Commitment Spectrum in T Cell Development

Author: Justin Le, Jeong Eun Park, Vi Luan Ha, Annie Luong, Sergio Branciamore, Andrei S. Rodin, Grigoriy Gogoshin, Fan Li, Yong-Hwee Eddie Loh, Virginia Camacho, Sweta B. Patel, Robert S. Welner, Chintan Parekh

Issue&Volume: 2020/06/16

Abstract: The challenges in recapitulating in vivo human T cell development in laboratory models have posed a barrier to understandinghuman thymopoiesis. Here, we used single-cell RNA sequencing (sRNA-seq) to interrogatethe rare CD34+ progenitor and the more differentiated CD34– fractions in the human postnatal thymus. CD34+ thymic progenitors were comprised of a spectrum of specification and commitment statescharacterized by multilineage priming followed by gradual T cell commitment. The earliestprogenitors in the differentiation trajectory were CD7– and expressed a stem-cell-like transcriptional profile, but had also initiated T cellpriming. Clustering analysis identified a CD34+ subpopulation primed for the plasmacytoid dendritic lineage, suggesting an intrathymicdendritic specification pathway. CD2 expression defined T cell commitment stages whereloss of B cell potential preceded that of myeloid potential. These datasets delineategene expression profiles spanning key differentiation events in human thymopoiesisand provide a resource for the further study of human T cell development.

DOI: 10.1016/j.immuni.2020.05.010

Source: https://www.cell.com/immunity/fulltext/S1074-7613(20)30218-1

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