小柯机器人

2020年7月6日，《自然—遗传学》杂志在线发表了美国科罗拉多大学Thomas R. Cech、John L. Rinn等研究人员的合作成果。他们的研究表明，RNA对于PRC2在人类多能干细胞中结合染色质以及发挥其功能至关重要。

Title: RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells

Author: Yicheng Long, Taeyoung Hwang, Anne R. Gooding, Karen J. Goodrich, John L. Rinn, Thomas R. Cech

Issue&Volume: 2020-07-06

Abstract: Many chromatin-binding proteins and protein complexes that regulate transcription also bind RNA. One of these, Polycomb repressive complex 2 (PRC2), deposits the H3K27me3 mark of facultative heterochromatin and is required for stem cell differentiation. PRC2 binds RNAs broadly in vivo and in vitro. Yet, the biological importance of this RNA binding remains unsettled. Here, we tackle this question in human induced pluripotent stem cells by using multiple complementary approaches. Perturbation of RNA–PRC2 interaction by RNase A, by a chemical inhibitor of transcription or by an RNA-binding-defective mutant all disrupted PRC2 chromatin occupancy and localization genome wide. The physiological relevance of PRC2–RNA interactions is further underscored by a cardiomyocyte differentiation defect upon genetic disruption. We conclude that PRC2 requires RNA binding for chromatin localization in human pluripotent stem cells and in turn for defining cellular state.

DOI: 10.1038/s41588-020-0662-x

Source: https://www.nature.com/articles/s41588-020-0662-x

Nature Genetics：《自然—遗传学》，创刊于1992年。隶属于施普林格·自然出版集团，最新IF：41.307
官方网址：https://www.nature.com/ng/
投稿链接：https://mts-ng.nature.com/cgi-bin/main.plex