小柯机器人

秀丽隐杆线虫规避病原体机制
2020-09-12 21:35

美国普林斯顿大学Coleen T. Murphy课题组取得一项新突破。他们的研究发现秀丽隐杆线虫解释细菌非编码RNA,以学习病原体规避。2020年9月9日,《自然》杂志发表了这一成果。

他们显示单次接触从致病性铜绿假单胞菌(PA14)中分离出的纯化小RNA,足以在蠕虫和四次传代中诱导病原体规避。RNA干扰(RNAi)和PIWI相互作用RNA(piRNA)途径、种系和ASI神经元都是细菌小RNA引起的规避行为以及此行为的跨代遗传所必需的。单个铜绿假单胞菌非编码RNA P11既是必要的也是充分的,以传递获得的对PA14的规避,而其秀丽隐杆线虫靶标maco-1也需要规避。

他们的结果表明,这种非编码RNA依赖的机制已演变为研究蠕虫的微生物环境,使用此信息做出适当的行为决策,并将此信息传递给子代。

据了解,由于营养食品暴露在多种细菌环境中,秀丽隐杆线虫必须从中区分出病原体。

附:英文原文

Title: C. elegans interprets bacterial non-coding RNAs to learn pathogenic avoidance

Author: Rachel Kaletsky, Rebecca S. Moore, Geoffrey D. Vrla, Lance R. Parsons, Zemer Gitai, Coleen T. Murphy

Issue&Volume: 2020-09-09

Abstract: Caenorhabditis elegans must distinguish pathogens from nutritious food sources among the many bacteria to which it is exposed in its environment1. Here we show that a single exposure to purified small RNAs isolated from pathogenic Pseudomonas aeruginosa (PA14) is sufficient to induce pathogen avoidance in the treated worms and in four subsequent generations of progeny. The RNA interference (RNAi) and PIWI-interacting RNA (piRNA) pathways, the germline and the ASI neuron are all required for avoidance behaviour induced by bacterial small RNAs, and for the transgenerational inheritance of this behaviour. A single P. aeruginosa non-coding RNA, P11, is both necessary and sufficient to convey learned avoidance of PA14, and its C. elegans target, maco-1, is required for avoidance. Our results suggest that this non-coding-RNA-dependent mechanism evolved to survey the microbial environment of the worm, use this information to make appropriate behavioural decisions and pass this information on to its progeny.

DOI: 10.1038/s41586-020-2699-5

Source: https://www.nature.com/articles/s41586-020-2699-5

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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