小柯机器人

美国亚利桑那州立大学陶农建和王少鹏研究团队提出了等离激元散射显微镜，实现了单个蛋白分子的结合动力学检测。 相关论文发表在2020年9月21日出版的《自然—方法学》杂志上。

研究团队提出了一种利用表面等离激元共振（SPR）散射技术测量单个蛋白结合动力学的方法。SPR由于具有非凡的灵敏度而成为一种流行的无标记检测技术，但从未用以成像单个蛋白质。研究人员通过成像蛋白的表面等离激元波的散射克服了这种限制。这使得他们实现成像单一蛋白质，测量蛋白的尺寸和基于特定的抗体结合识别蛋白。

该研究团队进一步表明，有可能通过计算单个分子的结合来量化蛋白质结合动力学，从而提供一种测量结合动力学和分析蛋白质行为异质性的数字方法。研究人员预计，这种成像方法将成为单个蛋白分子分析的一种重要工具，特别是对于低容量样本，例如单个细胞。

据介绍，测量单个蛋白质的结合动力学是蛋白质分析中最重要的并具有挑战性的任务之一。

附：英文原文

Title: Plasmonic scattering imaging of single proteins and binding kinetics

Author: Pengfei Zhang, Guangzhong Ma, Wei Dong, Zijian Wan, Shaopeng Wang, Nongjian Tao

Issue&Volume: 2020-09-21

Abstract: Measuring the binding kinetics of single proteins represents one of the most important and challenging tasks in protein analysis. Here we show that this is possible using a surface plasmon resonance (SPR) scattering technique. SPR is a popular label-free detection technology because of its extraordinary sensitivity, but it has never been used for imaging single proteins. We overcome this limitation by imaging scattering of surface plasmonic waves by proteins. This allows us to image single proteins, measure their sizes and identify them based on their specific binding to antibodies. We further show that it is possible to quantify protein binding kinetics by counting the binding of individual molecules, providing a digital method to measure binding kinetics and analyze heterogeneity of protein behavior. We anticipate that this imaging method will become an important tool for single protein analysis, especially for low volume samples, such as single cells. 

DOI: 10.1038/s41592-020-0947-0

Source: https://www.nature.com/articles/s41592-020-0947-0

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex