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突刺蛋白D614G突变可增强SARS-CoV-2传播性
2020-10-28 15:03

美国德克萨斯大学Pei-Yong Shi等研究人员发现,突刺蛋白D614G突变可增强SARS-CoV-2传播性。2020年10月26日,国际知名学术期刊《自然》在线发表了这一成果。

探究人员工程改造了USA-WA1/2020菌株中的D614G突变,并对其作用进行了表征。D614G通过增加病毒体的感染力来增强在人肺上皮细胞和主要人气道组织上的复制。感染了G614变体的仓鼠在鼻洗液和气管中产生了更高的感染滴度,但在肺部却没有,从而证实了临床证据,即D614G突变可增强COVID-19患者上呼吸道的病毒载量,并可能增加传播。
 
来自感染D614的仓鼠血清对G614病毒的中和效价略高于D614病毒,表明(i)该突变可能不会降低疫苗在临床试验中抵御COVID-19的能力,并且(ii)治疗性抗体应对G614病毒进行测试。结合临床发现,这项工作强调了这种突变在病毒传播、疫苗效力和抗体治疗中的重要性。
 
据了解,在COVID-19大流行期间,突刺蛋白突变D614G在SARS-CoV-2中占主导地位。但是,其对病毒传播和疫苗功效的影响尚待确定。
 
附:英文原文

Title: Spike mutation D614G alters SARS-CoV-2 fitness

Author: Jessica A. Plante, Yang Liu, Jianying Liu, Hongjie Xia, Bryan A. Johnson, Kumari G. Lokugamage, Xianwen Zhang, Antonio E. Muruato, Jing Zou, Camila R. Fontes-Garfias, Divya Mirchandani, Dionna Scharton, John P. Bilello, Zhiqiang Ku, Zhiqiang An, Birte Kalveram, Alexander N. Freiberg, Vineet D. Menachery, Xuping Xie, Kenneth S. Plante, Scott C. Weaver, Pei-Yong Shi

Issue&Volume: 2020-10-26

Abstract: A spike protein mutation D614G became dominant in SARS-CoV-2 during the COVID-19 pandemic1,2. However, the impact on viral spread and vaccine efficacy remains to be defined. Here, we engineer the D614G mutation in the USA-WA1/2020 strain and characterize its effect. D614G enhances replication on human lung epithelial cells and primary human airway tissues through an improved infectivity of virions. Hamsters infected with the G614 variant produced higher infectious titers in the nasal washes and trachea, but not lungs, confirming clinical evidence that the D614G mutation enhances viral loads in the upper respiratory tract of COVID-19 patients and may increases transmission. Sera from D614-infected hamsters exhibit modestly higher neutralization titers against G614 virus than against D614 virus, indicating that (i) the mutation may not reduce the ability of vaccines in clinical trials to protect against COVID-19 and (ii) therapeutic antibodies should be tested against the circulating G614 virus. Together with clinical findings, our work underscores the importance of this mutation in viral spread, vaccine efficacy, and antibody therapy.

DOI: 10.1038/s41586-020-2895-3

Source: https://www.nature.com/articles/s41586-020-2895-3

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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