小柯机器人

研究揭示小鼠运动皮层转录组细胞类型的表型变异
2020-11-16 13:56

美国贝勒医学院Andreas S. Tolias、德国图宾根大学Philipp Berens等研究人员合作揭示小鼠运动皮层转录组细胞类型的表型变异。相关论文于2020年11月12日在线发表于国际学术期刊《自然》。

研究人员使用Patch-seq并结合膜片钳记录、生物胞素染色和成年小鼠原代运动皮层中1,300多个神经元的单细胞RNA测序,来提供了几乎所有转录组定义神经细胞类型的形-电注释。研究人员发现,尽管转录组类型的广泛家族(表达Vip、Pvalb,Sst等的家族)具有截然不同且基本不重叠的形-电表型,但同一家族中的各个转录组类型在形-电空间中并未很好地分离。取而代之的是,形态和电生理学具有连续性的变化,相邻的转录组细胞类型显示出相似的形-电特征,它们之间通常没有明确的界限。
 
这些结果表明,新皮层中的神经元类型并不总是形成离散实体。取而代之的是,神经元形成了一个层次结构,该层次结构由家族级别的不同的非重叠分支组成,但可以在家族内部形成连续且相关的转录组和形-电图谱。
 
据介绍,皮质神经元在基因表达以及形态和电生理特性上表现出极大的多样性。大多数现有的神经分类法都基于转录组或电生理性质,因为在同一组细胞中研究神经元多样性的两个方面在技术上都具有挑战性。
 
附:英文原文

Title: Phenotypic variation of transcriptomic cell types in mouse motor cortex

Author: Federico Scala, Dmitry Kobak, Matteo Bernabucci, Yves Bernaerts, Cathryn Ren Cadwell, Jesus Ramon Castro, Leonard Hartmanis, Xiaolong Jiang, Sophie Laturnus, Elanine Miranda, Shalaka Mulherkar, Zheng Huan Tan, Zizhen Yao, Hongkui Zeng, Rickard Sandberg, Philipp Berens, Andreas S. Tolias

Issue&Volume: 2020-11-12

Abstract: Cortical neurons exhibit extreme diversity in gene expression as well as in morphological and electrophysiological properties1,2. Most existing neural taxonomies are based on either transcriptomic3,4 or morpho-electric5,6 criteria, as it has been technically challenging to study both aspects of neuronal diversity in the same set of cells7. Here we used Patch-seq8 to combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of more than 1,300 neurons in adult mouse primary motor cortex, providing a morpho-electric annotation of almost all transcriptomically defined neural cell types. We found that, although broad families of transcriptomic types (those expressing Vip, Pvalb, Sst and so on) had distinct and essentially non-overlapping morpho-electric phenotypes, individual transcriptomic types within the same family were not well separated in the morpho-electric space. Instead, there was a continuum of variability in morphology and electrophysiology, with neighbouring transcriptomic cell types showing similar morpho-electric features, often without clear boundaries between them. Our results suggest that neuronal types in the neocortex do not always form discrete entities. Instead, neurons form a hierarchy that consists of distinct non-overlapping branches at the level of families, but can form continuous and correlated transcriptomic and morpho-electrical landscapes within families. Single-cell transcriptomic, morphological and electrophysiological characteristics are combined to classify more than 1,300 neurons from mouse motor cortex.

DOI: 10.1038/s41586-020-2907-3

Source: https://www.nature.com/articles/s41586-020-2907-3

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:69.504
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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