小柯机器人

近日，德国弗莱堡大学Christoph Neumann-Haefelin等研究人员合作揭示了预先存在和诱导的SARS-CoV-2特异性CD8⁺T细胞特征。2020年11月12日，《自然—医学》杂志在线发表了这项成果。

Title: Characterization of pre-existing and induced SARS-CoV-2-specific CD8 + T cells

Author: Isabel Schulien, Janine Kemming, Valerie Oberhardt, Katharina Wild, Lea M. Seidel, Saskia Killmer, Sagar, Franziska Daul, Marilyn Salvat Lago, Annegrit Decker, Hendrik Luxenburger, Benedikt Binder, Dominik Bettinger, Oezlem Sogukpinar, Siegbert Rieg, Marcus Panning, Daniela Huzly, Martin Schwemmle, Georg Kochs, Cornelius F. Waller, Alexandra Nieters, Daniel Duerschmied, Florian Emmerich, Henrik E. Mei, Axel Ronald Schulz, Sian Llewellyn-Lacey, David A. Price, Tobias Boettler, Bertram Bengsch, Robert Thimme, Maike Hofmann, Christoph Neumann-Haefelin

Issue&Volume: 2020-11-12

Abstract: Emerging data indicate that SARS-CoV-2-specific CD8+ T cells targeting different viral proteins are detectable in up to 70% of convalescent individuals1,2,3,4,5. However, very little information is currently available about the abundance, phenotype, functional capacity and fate of pre-existing and induced SARS-CoV-2-specific CD8+ T cell responses during the natural course of SARS-CoV-2 infection. Here, we define a set of optimal and dominant SARS-CoV-2-specific CD8+ T cell epitopes. We also perform a high-resolution ex vivo analysis of pre-existing and induced SARS-CoV-2-specific CD8+ T cells, applying peptide-loaded major histocompatibility complex class I (pMHCI) tetramer technology. We observe rapid induction, prolonged contraction and emergence of heterogeneous and functionally competent cross-reactive and induced memory CD8+ T cell responses in cross-sectionally analyzed individuals with mild disease following SARS-CoV-2 infection and three individuals longitudinally assessed for their T cells pre- and post-SARS-CoV-2 infection. SARS-CoV-2-specific memory CD8+ T cells exhibited functional characteristics comparable to influenza-specific CD8+ T cells and were detectable in SARS-CoV-2 convalescent individuals who were seronegative for anti-SARS-CoV-2 antibodies targeting spike (S) and nucleoprotein (N). These results define cross-reactive and induced SARS-CoV-2-specific CD8+ T cell responses as potentially important determinants of immune protection in mild SARS-CoV-2 infection.

DOI: 10.1038/s41591-020-01143-2

Source: https://www.nature.com/articles/s41591-020-01143-2

Nature Medicine：《自然—医学》，创刊于1995年。隶属于施普林格·自然出版集团，最新IF：87.241
官方网址：https://www.nature.com/nm/
投稿链接：https://mts-nmed.nature.com/cgi-bin/main.plex