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研究揭示光合反应中心会发生超快结构变化
2020-12-03 15:52

瑞典哥德堡大学Richard Neutze研究小组揭示,光合反应中心会发生超快结构变化。该研究于2020年12月2号在线发表于《自然》杂志。

在本研究中,研究人员使用时间分辨的串联飞秒晶体学、X射线自由电子激光,在皮秒级的时间尺度上观察了绿藻中光合作用反应中心由光诱导的结构变化。结构扰动首先发生在光合作用中心的一对特殊叶绿素分子上,其在光合作用过程中发生光氧化。将电子转移到膜另一侧的甲萘醌受体上会引起该辅因子的运动,并导致振幅较低的蛋白质重排。这些结果揭示了蛋白质如何利用构象动力学来稳定电子转移过程中的电荷分离。

研究人员表示,光合作用反应中心通过能量转换生物膜传递电子来收集光能量。

附:英文原文

Title: Ultrafast structural changes within a photosynthetic reaction centre

Author: Robert Dods, Petra Bth, Dmitry Morozov, Viktor Ahlberg Gagnr, David Arnlund, Hoi Ling Luk, Joachim Kbel, Micha Maj, Adams Vallejos, Cecilia Wickstrand, Robert Bosman, Kenneth R. Beyerlein, Garrett Nelson, Mengning Liang, Despina Milathianaki, Joseph Robinson, Rajiv Harimoorthy, Peter Berntsen, Erik Malmerberg, Linda Johansson, Rebecka Andersson, Sergio Carbajo, Elin Claesson, Chelsie E. Conrad, Peter Dahl, Greger Hammarin, Mark S. Hunter, Chufeng Li, Stella Lisova, Antoine Royant, Cecilia Safari, Amit Sharma, Garth J. Williams, Oleksandr Yefanov, Sebastian Westenhoff, Jan Davidsson, Daniel P. DePonte, Sbastien Boutet, Anton Barty, Gergely Katona, Gerrit Groenhof, Gisela Brndn, Richard Neutze

Issue&Volume: 2020-12-02

Abstract: Photosynthetic reaction centres harvest the energy content of sunlight by transporting electrons across an energy-transducing biological membrane. Here we use time-resolved serial femtosecond crystallography1 using an X-ray free-electron laser2 to observe light-induced structural changes in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds. Structural perturbations first occur at the special pair of chlorophyll molecules of the photosynthetic reaction centre that are photo-oxidized by light. Electron transfer to the menaquinone acceptor on the opposite side of the membrane induces a movement of this cofactor together with lower amplitude protein rearrangements. These observations reveal how proteins use conformational dynamics to stabilize the charge-separation steps of electron-transfer reactions. Time-resolved serial femtosecond crystallography is used to reveal the structural changes that stabilize the charge-separation steps of electron-transfer reactions in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds.

DOI: 10.1038/s41586-020-3000-7

Source: https://www.nature.com/articles/s41586-020-3000-7

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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