小柯机器人

韩国先进科技学院Eui-Cheol Shin等研究人员合作发现，PD-1表达的SARS-CoV-2特异性CD8⁺T细胞并未耗竭，并在COVID-19患者中起作用。2020年12月10日，《免疫》杂志在线发表了这项成果。

研究人员通过MHC-1多聚体染色检测到SARS-CoV-2特异性CD8⁺T细胞，并检查了它们在急性和恢复期COVID-19中的表型和功能。在恢复期早期，多聚体⁺细胞表现出早期分化的效应记忆表型。在恢复期后期，多聚体⁺细胞中干细胞样记忆细胞的频率增加。与MHC-1多聚体染色相结合的细胞因子分泌测定表明，在SARS-CoV-2特异性CD8⁺T细胞中，产生干扰素-γ（IFN-γ）的细胞的比例明显低于对甲型流感病毒特异性的细胞。

重要的是，多聚体⁺细胞中PD-1⁺细胞中产生IFN-γ的细胞比例高于PD-1^-细胞，这表明表达PD-1的SARS-CoV-2特异性CD8⁺T细胞并未耗尽，且功能正常。这些发现为理解感染或接种疫苗引发的SARS-CoV-2特异性CD8⁺T细胞提供了信息。

据了解，记忆T细胞反应已在COVID-19恢复期中被证明，但SARS-CoV-2特异性T细胞的离体表型尚不清楚。

附：英文原文

Title: PD-1-expressing SARS-CoV-2-specific CD8+ T Cells Are Not Exhausted, but Functional in Patients with COVID-19

Author: Min-Seok Rha, Hye Won Jeong, Jae-Hoon Ko, Seong Jin Choi, In-Ho Seo, Jeong Seok Lee, Moa Sa, A Reum Kim, Eun-Jeong Joo, Jin Young Ahn, Jung Ho Kim, Kyoung-Ho Song, Eu Suk Kim, Dong Hyun Oh, Mi Young Ahn, Hee Kyoung Choi, Ji Hoon Jeon, Jae-Phil Choi, Hong Bin Kim, Young Keun Kim, Su-Hyung Park, Won Suk Choi, Jun Yong Choi, Kyong Ran Peck, Eui-Cheol Shin

Issue&Volume: 2020-12-10

Abstract: Memory T cell responses have been demonstrated in COVID-19 convalescents, but ex vivo phenotypes of SARS-CoV-2-specific T cells have been unclear. We detected SARS-CoV-2-specific CD8+ T cells by MHC-I multimer staining and examined their phenotypes and functions in acute and convalescent COVID-19. Multimer+ cells exhibited early differentiated effector-memory phenotypes in the early convalescent phase. The frequency of stem-like memory cells was increased among multimer+ cells in the late convalescent phase. Cytokine secretion assays combined with MHC-I multimer staining revealed that the proportion of interferon-γ (IFN-γ)-producing cells was significantly lower among SARS-CoV-2-specific CD8+ T cells than those specific to influenza A virus. Importantly, the proportion of IFN-γ-producing cells was higher in PD-1+ cells than PD-1- cells among multimer+ cells, indicating that PD-1-expressing, SARS-CoV-2-specific CD8+ T cells are not exhausted, but functional. Our current findings provide information for understanding of SARS-CoV-2-specific CD8+ T cells elicited by infection or vaccination.

DOI: 10.1016/j.immuni.2020.12.002

Source: https://www.cell.com/immunity/fulltext/S1074-7613(20)30509-4

Immunity：《免疫》，创刊于1994年。隶属于细胞出版社，最新IF：43.474
官方网址：https://www.cell.com/immunity/home
投稿链接：https://www.editorialmanager.com/immunity/default.aspx