小柯机器人

南丹麦大学Susanne Mandrup、Jesper Grud Skat Madsen等研究人员合作揭示在单核分辨率下附睾脂肪组织响应饮食诱导肥胖时的可塑性。2020年12月29日，《细胞—代谢》杂志在线发表了这项成果。

研究人员利用单核RNA-seq来绘制了小鼠附睾白色脂肪组织在高脂肪饮食诱导的肥胖反应下的可塑性。单核方法使研究人员能够解析所有的主要细胞类型，并揭示了从成脂前细胞到成熟成脂细胞整个成脂轨迹的独特转录阶段。

研究人员证明了不同的脂肪细胞亚群的存在，并表明肥胖导致脂肪生成亚群的消失和应激脂质清除亚群的丰度增加。此外，肥胖症与其他细胞群的丰度和基因表达的重大变化有关，包括巨噬细胞特异性基因的下调情况下脂质处理基因的急剧增加。这些数据为将来的脂肪细胞分化和脂肪组织可塑性研究提供了有力的资源。

据了解，脂肪组织显示出显著的饮食状态适应能力。

附：英文原文

Title: Plasticity of Epididymal Adipose Tissue in Response to Diet-Induced Obesity at Single-Nucleus Resolution

Author: Anitta Kinga Sárvári, Elvira Laila Van Hauwaert, Lasse Kruse Markussen, Ellen Gammelmark, Ann-Britt Marcher, Morten Frend Ebbesen, Ronni Nielsen, Jonathan Richard Brewer, Jesper Grud Skat Madsen, Susanne Mandrup

Issue&Volume: 2020-12-29

Abstract: Adipose tissues display a remarkable ability to adapt to the dietary status. Here,we have applied single-nucleus RNA-seq to map the plasticity of mouse epididymal whiteadipose tissue at single-nucleus resolution in response to high-fat-diet-induced obesity.The single-nucleus approach allowed us to recover all major cell types and to revealdistinct transcriptional stages along the entire adipogenic trajectory from preadipocytecommitment to mature adipocytes. We demonstrate the existence of different adipocytesubpopulations and show that obesity leads to disappearance of the lipogenic subpopulationand increased abundance of the stressed lipid-scavenging subpopulation. Moreover,obesity is associated with major changes in the abundance and gene expression of othercell populations, including a dramatic increase in lipid-handling genes in macrophagesat the expense of macrophage-specific genes. The data provide a powerful resourcefor future hypothesis-driven investigations of the mechanisms of adipocyte differentiationand adipose tissue plasticity.

DOI: 10.1016/j.cmet.2020.12.004

Source: https://www.cell.com/cell-metabolism/fulltext/S1550-4131(20)30659-8

Cell Metabolism：《细胞—代谢》，创刊于2005年。隶属于细胞出版社，最新IF：31.373
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