小柯机器人

德国雷根斯堡大学Markus Feuerer小组利用单细胞染色质可及性测序发现人类调节性T细胞中的组织修复程序。相关论文于2021年3月30日在线发表在《免疫》杂志上。

研究人员确定了在健康组织中人类调节性T（Treg）的细胞特征。小鼠和人类组织Treg细胞的单细胞染色质可及性谱定义了一个保守的、不依赖微生物群的组织修复Treg标记，并且转录因子BATF占主导的地位。将这种特征与基因表达谱分析和TCR命运图谱相结合，研究人员在人外周血中鉴定出一群表达BATF、趋化因子受体CCR8和HLA-DR的组织样Treg细胞。

正常皮肤和脂肪组织中的人BATF⁺CCR8⁺Treg细胞与非淋巴T滤泡辅助样（Tfh-like）细胞具有共同特征，并且在初始的人Treg细胞中诱导Tfh-样分化程序部分重现了组织Treg的再生特性，包括伤口愈合潜力。来自人类健康组织的BATF⁺CCR8⁺Treg细胞与驻留于肿瘤的Treg细胞具有共同的特征，这突显了理解这些细胞背景特异性功能的重要性。 

据悉，小鼠组织中的Treg细胞促进组织稳态和再生。

附：英文原文

Title: Single-cell chromatin accessibility landscape identifies tissue repair program in human regulatory T cells

Author: Michael Delacher, Malte Simon, Lieke Sanderink, Agnes Hotz-Wagenblatt, Marina Wuttke, Kathrin Schambeck, Lisa Schmidleithner, Sebastian Bittner, Asmita Pant, Uwe Ritter, Thomas Hehlgans, Dania Riegel, Verena Schneider, Florian Kai Groeber-Becker, Andreas Eigenberger, Claudia Gebhard, Nicholas Strieder, Alexander Fischer, Michael Rehli, Petra Hoffmann, Matthias Edinger, Till Strowig, Jochen Huehn, Christian Schmidl, Jens M. Werner, Lukas Prantl, Benedikt Brors, Charles D. Imbusch, Markus Feuerer

Issue&Volume: 2021-03-30

Abstract: Murine regulatory T (Treg) cells in tissues promote tissue homeostasis and regeneration. We sought to identify features that characterize human Treg cells with these functions in healthy tissues. Single-cell chromatin accessibility profiles of murine and human tissue Treg cells defined a conserved, microbiota-independent tissue-repair Treg signature with a prevailing footprint of the transcription factor BATF. This signature, combined with gene expression profiling and TCR fate mapping, identified a population of tissue-like Treg cells in human peripheral blood that expressed BATF, chemokine receptor CCR8 and HLA-DR. Human BATF+CCR8+ Treg cells from normal skin and adipose tissue shared features with nonlymphoid T follicular helper-like (Tfh-like) cells, and induction of a Tfh-like differentiation program in naive human Treg cells partially recapitulated tissue Treg regenerative characteristics, including wound healing potential. Human BATF+CCR8+ Treg cells from healthy tissue share features with tumor-resident Treg cells, highlighting the importance of understanding the context-specific functions of these cells.

DOI: 10.1016/j.immuni.2021.03.007

Source: https://www.cell.com/immunity/fulltext/S1074-7613(21)00119-9

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