小柯机器人

新型生物传感器能够直接可视化生长素
2021-04-11 18:08

德国马克斯-普朗克发育生物学研究所Gerd Jürgens、Birte Höcker等研究人员合作开发出一种能够直接可视化生长素的生物传感器。2021年4月7日,《自然》杂志在线发表了这项成果。

研究人员报道了一种遗传编码的生物传感器,可用于体内生长素分布的定量可视化。该传感器基于大肠杆菌色氨酸抑制因子,其结合口袋被设计成对生长素具有特异性。生长素结合部分与荧光蛋白的偶联使得能够使用荧光共振能量转移信号作为读数。与以前的系统不同,该传感器可以直接监测单个细胞以及植物体内细胞内生长素的快速摄取和清除。通过响应沿根轴的渐变空间分布及其受到运输抑制剂的扰动,以及响应重力矢量的变化时,内源性生长素快速而可逆地重新分布,这个传感器可以在植物的生命周期内以亚细胞分辨率实时监测植物生长素浓度及其在空间和时间上的变化。 

据介绍,植物中最重要的调节性小分子之一是吲哚-3-乙酸,也称为生长素。它的动态重新分布在植物生命的几乎每个方面都具有至关重要的作用,从细胞形状和分裂到器官发生以及对光和重力的响应。迄今为止,尚不可能以细胞分辨率直接确定植物生长素的时空分布。相反,这是从内源性生长素响应机器的分布推断出来的,但是,这样的系统无法检测到瞬态变化。

附:英文原文

Title: A biosensor for the direct visualization of auxin

Author: Ole Herud-Sikimi, Andre C. Stiel, Martina Kolb, Sooruban Shanmugaratnam, Kenneth W. Berendzen, Christian Feldhaus, Birte Hcker, Gerd Jrgens

Issue&Volume: 2021-04-07

Abstract: One of the most important regulatory small molecules in plants is indole-3-acetic acid, also known as auxin. Its dynamic redistribution has an essential role in almost every aspect of plant life, ranging from cell shape and division to organogenesis and responses to light and gravity1,2. So far, it has not been possible to directly determine the spatial and temporal distribution of auxin at a cellular resolution. Instead it is inferred from the visualization of irreversible processes that involve the endogenous auxin-response machinery3,4,5,6,7; however, such a system cannot detect transient changes. Here we report a genetically encoded biosensor for the quantitative in vivo visualization of auxin distribution. The sensor is based on the Escherichia coli tryptophan repressor8, the binding pocket of which is engineered to be specific to auxin. Coupling of the auxin-binding moiety with selected fluorescent proteins enables the use of a fluorescence resonance energy transfer signal as a readout. Unlike previous systems, this sensor enables direct monitoring of the rapid uptake and clearance of auxin by individual cells and within cell compartments in planta. By responding to the graded spatial distribution along the root axis and its perturbation by transport inhibitors—as well as the rapid and reversible redistribution of endogenous auxin in response to changes in gravity vectors—our sensor enables real-time monitoring of auxin concentrations at a (sub)cellular resolution and their spatial and temporal changes during the lifespan of a plant.

DOI: 10.1038/s41586-021-03425-2

Source: https://www.nature.com/articles/s41586-021-03425-2

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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