小柯机器人

刚果共和国国家生物医学研究所Eddy Kinganda-Lusamaki等研究人员合作揭示基因组测序对埃博拉病毒爆发应对措施的价值。该项研究成果于2021年4月12日在线发表在《自然—医学》杂志上。

据研究人员介绍，2018年8月1日，刚果民主共和国（DRC）宣布其第十次埃博拉病毒病（EVD）爆发。为了帮助流行病学应对，国家生物医学研究所（INRB）实施了端到端的基因组监测系统，包括测序、生物信息学分析和向一线公共卫生工作者传播基因组流行病学结果。

研究人员报告了由这项监测工作产生的744个新基因组，采样时间为2018年7月27日至2020年4月27日。连同先前可获得的序列数据（n=48个基因组），这些数据占分析期间DRC中所有实验室确诊的埃博拉病毒（EBOV）感染的近24％。随着新序列的产生，研究人员从基因组数据推断出时空传播动态，并发布了这些结果来支持流行病学应对工作。研究人员概述了该基因组监测系统的功能，对北基伍省爆发的792个埃博拉病毒基因组进行了完整的系统动力学分析，并讨论了基因组监测数据如何为应对工作和公共卫生决策提供依据。 

附：英文原文

Title: Integration of genomic sequencing into the response to the Ebola virus outbreak in Nord Kivu, Democratic Republic of the Congo

Author: Eddy Kinganda-Lusamaki, Allison Black, Daniel B. Mukadi, James Hadfield, Placide Mbala-Kingebeni, Catherine B. Pratt, Amuri Aziza, Moussa M. Diagne, Bailey White, Nella Bisento, Bibiche Nsunda, Marceline Akonga, Martin Faye, Ousmane Faye, Francois Edidi-Atani, Meris Matondo-Kuamfumu, Fabrice Mambu-Mbika, Junior Bulabula, Nicholas Di Paola, Matthias G. Pauthner, Kristian G. Andersen, Gustavo Palacios, Eric Delaporte, Amadou Alpha Sall, Martine Peeters, Michael R. Wiley, Steve Ahuka-Mundeke, Trevor Bedford, Jean-Jacques Muyembe Tamfum

Issue&Volume: 2021-04-12

Abstract: On 1August 2018, the Democratic Republic of the Congo (DRC) declared its tenth Ebola virus disease (EVD) outbreak. To aid the epidemiologic response, the Institut National de Recherche Biomédicale (INRB) implemented an end-to-end genomic surveillance system, including sequencing, bioinformatic analysis and dissemination of genomic epidemiologic results to frontline public health workers. We report 744new genomes sampled between 27July 2018 and 27April 2020 generated by this surveillance effort. Together with previously available sequence data (n=48 genomes), these data represent almost 24% of all laboratory-confirmed Ebola virus (EBOV) infections in DRC in the period analyzed. We inferred spatiotemporal transmission dynamics from the genomic data as new sequences were generated, and disseminated the results to support epidemiologic response efforts. Here we provide an overview of how this genomic surveillance system functioned, present a full phylodynamic analysis of 792Ebola genomes from the Nord Kivu outbreak and discuss how the genomic surveillance data informed response efforts and public health decision making.

DOI: 10.1038/s41591-021-01302-z

Source: https://www.nature.com/articles/s41591-021-01302-z

Nature Medicine：《自然—医学》，创刊于1995年。隶属于施普林格·自然出版集团，最新IF：87.241
官方网址：https://www.nature.com/nm/
投稿链接：https://mts-nmed.nature.com/cgi-bin/main.plex