小柯机器人

通过体细胞突变追踪人类发育谱系
2021-05-16 15:42

英国威康信托桑格研究所Ana Cvejic和Peter J. Campbell研究团队合作取得最新进展。他们通过体细胞突变追踪人类发育谱系。这一研究成果发表在2021年5月12日出版的国际学术期刊《自然》杂志上。

他们在受孕后第8周和第18周,使用来自健康人类胎儿的511个单细胞来源的造血集落的全基因组测序,并结合已知胚胎起源组织的深度靶向测序,重建了血液发育的系统树。他们发现,在健康的胎儿中,单个造血祖细胞在受孕后18周内会获得数十个体细胞突变。他们将这些突变用作条形码,并对发育过程中胚胎和胚外组织的发散时间进行计时,并估算了胚胎发育不同阶段的血液前体数量。他们的数据支持人胚外中胚层和原始血液的成胚起源。

据介绍,胎儿发育过程中人类造血系统的个体发育以前主要是通过仔细的显微镜观察来表征的。

附:英文原文

Title: Lineage tracing of human development through somatic mutations

Author: Michael Spencer Chapman, Anna Maria Ranzoni, Brynelle Myers, Nicholas Williams, Tim H. H. Coorens, Emily Mitchell, Timothy Butler, Kevin J. Dawson, Yvette Hooks, Luiza Moore, Jyoti Nangalia, Philip S. Robinson, Kenichi Yoshida, Elizabeth Hook, Peter J. Campbell, Ana Cvejic

Issue&Volume: 2021-05-12

Abstract: The ontogeny of the human haematopoietic system during fetal development has previously been characterized mainly through careful microscopic observations1. Here we reconstruct a phylogenetic tree of blood development using whole-genome sequencing of 511 single-cell-derived haematopoietic colonies from healthy human fetuses at 8 and 18 weeks after conception, coupled with deep targeted sequencing of tissues of known embryonic origin. We found that, in healthy fetuses, individual haematopoietic progenitors acquire tens of somatic mutations by 18 weeks after conception. We used these mutations as barcodes and timed the divergence of embryonic and extra-embryonic tissues during development, and estimated the number of blood antecedents at different stages of embryonic development. Our data support a hypoblast origin of the extra-embryonic mesoderm and primitive blood in humans.

DOI: 10.1038/s41586-021-03548-6

Source: https://www.nature.com/articles/s41586-021-03548-6

Nature:《自然》,创刊于1869年。隶属于施普林格·自然出版集团,最新IF:43.07
官方网址:http://www.nature.com/
投稿链接:http://www.nature.com/authors/submit_manuscript.html


本期文章:《自然》:Online/在线发表

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