小柯机器人

德国明斯特大学医院Gerd Meyer zu Horste及其研究团队表明CNS 边界区室白细胞的单细胞分析表明 B 细胞及其祖细胞位于非患病脑膜中。2021年7月12日出版的《自然—神经科学》发表了这项成果。

基于单细胞转录组学，他们确定了中枢神经系统实质、富含软脑膜的硬膜下脑膜、硬脑膜、脉络丛和脑脊液中组织驻留白细胞的高度位置特异性组成和表达谱。在小鼠和大鼠的稳态条件下，脑膜的硬脑膜层含有大量 B 细胞。鼠硬脑膜 B 细胞表现出缓慢的更新和长期的组织驻留，并且它们在实验性神经炎症中成熟。硬脑膜还包含在 pro-B 细胞阶段的 B 谱系祖细胞，通常在骨髓外找不到，不会从外周或颅骨骨髓直接流入。这将硬脑膜确定为一个意外的 B 细胞驻留位点，并可能在体内稳态和神经炎症中发展。

据介绍，中枢神经系统被脑膜和脑脊液包裹，最近的研究结果表明这些中枢神经系统相关的边界组织具有复杂的免疫功能。与髓系细胞不同，边界区室中的淋巴细胞尚未完全表征。

附：英文原文

Title: Single-cell profiling of CNS border compartment leukocytes reveals that B cells and their progenitors reside in non-diseased meninges

Author: David Schafflick, Jolien Wolbert, Michael Heming, Christian Thomas, Maike Hartlehnert, Anna-Lena Brsch, Alessio Ricci, Sandra Martn-Salamanca, Xiaolin Li, I-Na Lu, Mathias Pawlak, Jens Minnerup, Jan-Kolja Strecker, Thomas Seidenbecher, Sven G. Meuth, Andres Hidalgo, Arthur Liesz, Heinz Wiendl, Gerd Meyer zu Horste

Issue&Volume: 2021-07-12

Abstract: The CNS is ensheathed by the meninges and cerebrospinal fluid, and recent findings suggest that these CNS-associated border tissues have complex immunological functions. Unlike myeloid lineage cells, lymphocytes in border compartments have yet to be thoroughly characterized. Based on single-cell transcriptomics, we here identified a highly location-specific composition and expression profile of tissue-resident leukocytes in CNS parenchyma, pia-enriched subdural meninges, dura mater, choroid plexus and cerebrospinal fluid. The dura layer of the meninges contained a large population of B cells under homeostatic conditions in mice and rats. Murine dura B cells exhibited slow turnover and long-term tissue residency, and they matured in experimental neuroinflammation. The dura also contained B lineage progenitors at the pro-B cell stage typically not found outside of bone marrow, without direct influx from the periphery or the skull bone marrow. This identified the dura as an unexpected site of B cell residence and potentially of development in both homeostasis and neuroinflammation. The skull dura contains B cells and B lineage precursors under homeostatic conditions. These cells are long-term tissue resident and mature upon neuroinflammation. This identifies the dura as a site of B cell residence and potentially development.

DOI: 10.1038/s41593-021-00880-y

Source: https://www.nature.com/articles/s41593-021-00880-y

Nature Neuroscience：《自然—神经科学》，创刊于1998年。隶属于施普林格·自然出版集团，最新IF：28.771
官方网址：https://www.nature.com/neuro/
投稿链接：https://mts-nn.nature.com/cgi-bin/main.plex