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不同的人类朗格汉斯细胞亚群协调相互的功能
2021-09-17 13:31

复旦大学李巍等研究人员合作发现,不同的人类朗格汉斯细胞亚群协调相互的功能。该研究于2021年9月10日在线发表于国际一流学术期刊《免疫》。

通过单细胞RNA测序和质谱技术,研究人员在人类皮肤的表皮和CD34+造血干细胞衍生的朗格汉斯细胞(HSC-LC)中确定了两个稳态(LC1和LC2)和两个激活的LC亚群。对分化过程中多个时间点造血干细胞的分析显示,EGR1和Notch信号是调节LC1和LC2分叉的主要途径之一。LC1被描述为经典的LC,主要与先天免疫和抗原处理有关。LC2类似于单核细胞或骨髓树突细胞,参与免疫反应和白细胞活化。LC1在炎症微环境下保持稳定,而LC2则容易被激活,并表现出免疫抑制分子的高表达。

研究人员揭示了不同的人类LC亚群,它们需要不同的发育调节并协调相互的功能。

据介绍,LC在皮肤稳态中起着关键作用,而LC的异质性长期以来一直被认为是一个问题。

附:英文原文

Title: Distinct human Langerhans cell subsets orchestrate reciprocal functions and require different developmental regulation

Author: Xiaochun Liu, Ronghui Zhu, Yang Luo, Shangshang Wang, Yi Zhao, Zhuoqiong Qiu, Yu Zhang, Xiao Liu, Xu Yao, Xiao Li, Wei Li

Issue&Volume: 2021-09-10

Abstract: Langerhans cells (LCs) play a pivotal role in skin homeostasis, and the heterogeneityof LCs has long been considered. In this study, we have identified two steady-state(LC1 and LC2) and two activated LC subsets in the epidermis of human skin and in LCsderived from CD34+ hemopoietic stem cells (HSC-LCs) by utilizing single-cell RNA sequencing and masscytometry. Analysis of HSC-LCs at multiple time-points during differentiation revealedthat EGR1 and Notch signaling were among the top pathways regulating the bifurcationof LC1 and LC2. LC1 were characterized as classical LCs, mainly related to innateimmunity and antigen processing. LC2 were similar to monocytes or myeloid dendriticcells, involving in immune responses and leukocyte activation. LC1 remained stableunder inflammatory microenvironment, whereas LC2 were prone to being activated anddemonstrated elevated expression of immuno-suppressive molecules. We revealed distincthuman LC subsets that require different developmental regulation and orchestrate reciprocal functions.

DOI: 10.1016/j.immuni.2021.08.012

Source: https://www.cell.com/immunity/fulltext/S1074-7613(21)00344-7

Immunity:《免疫》,创刊于1994年。隶属于细胞出版社,最新IF:43.474
官方网址:https://www.cell.com/immunity/home
投稿链接:https://www.editorialmanager.com/immunity/default.aspx


本期文章:《免疫》:Online/在线发表

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