小柯机器人

5′-Phosphorylation Strengthens Sticky-End Cohesions
2021-09-15 16:10

美国普渡大学Chengde Mao团队报道了5 '' -磷酸化增强粘端内聚。相关研究成果于2021年9月13日发表在《美国化学会杂志》。

粘端内聚在分子生物学和核酸纳米技术中起着至关重要的作用。虽然自由能计算和分子力学可以预测这些相互作用,但化学修饰会影响这些预测。

该文中,研究人员使用合理设计的3D DNA晶体作为工具,实验研究5′-磷酸化对粘端内聚的调节作用。研究人员发现5′-磷酸化增强了粘端内聚:在完全通过粘端内聚自组装的DNA晶体中,5′-磷酸化不仅促进了结晶过程,而且还加速了晶体沿设计方向的生长。

该发现使得DNA结晶动力学的微调和DNA晶体形态的控制成为可能,还表明天然DNA(具有5′-磷酸化)和合成DNA(不具有5′-磷酸化)之间的自组装动力学存在潜在差异。

附:英文原文

Title: 5′-Phosphorylation Strengthens Sticky-End Cohesions

Author: Zhe Li, Mengxi Zheng, Longfei Liu, Nadrian C. Seeman, Chengde Mao

Issue&Volume: September 13, 2021

Abstract: Sticky-end cohesion plays a critical role in molecular biology and nucleic acid nanotechnology. Although free energy calculations and molecular mechanics can predict these interactions, chemical modification would compromise such predictions. Herein, we have used rationally designed 3D DNA crystals as a tool to experimentally investigate the modulation of 5′-phosphorylation on sticky-end cohesions. We have found that 5′-phosphorylation strengthens the sticky-end cohesion: in a DNA crystal self-assembled exclusively via sticky-end cohesions, 5′-phosphorylation not only promotes the crystallization process, in general, but also accelerates the crystal growth along designed directions. Such a finding allows the fine-tuning of DNA crystallization kinetics and the control of DNA crystal morphology. It also suggests a potential difference in self-assembly kinetics between natural DNA (with 5′-phosphorylation) and synthetic DNA (without 5′-phosphorylation).

DOI: 10.1021/jacs.1c07279

Source: https://pubs.acs.org/doi/10.1021/jacs.1c07279

 

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