中国大连化学物理研究所徐兆超团队通过氢键敏感荧光探针的缓冲策略对脂滴动力学进行稳定的超分辨率成像。相关研究成果于2021年9月14日发表在《德国应用化学》。
尽管超分辨率成像提供了在纳米级可视化细胞结构和细胞器的机会，但细胞异质性和不可预测性仍然是活细胞动态成像的一个重大挑战。因此，为长期超分辨率成像开发性能更好、更具光稳定性的探头至关重要。
该文中，研究人员报告了一种使用结构照明显微镜（SIM）成像脂滴（LD）动力学的探针LD-FG。由于氢键敏感的荧光性，LD-FG能够使LDs免清洗成像。LDs外的完整LD-FG对光漂白LD-FG的补充进一步保证了荧光成像的长期稳定性。有了该缓冲荧光探针，LDs的快速和不可预测的动态过程可以可视化。研究人员首次发现了两种LD聚结模式（以及细胞不同区域甚至不同细胞之间的异质性）。
值得注意的是，动态成像帮助研究人员提出脂肪细胞分化过程中LD成熟的新模型，即快速LD合并后缓慢成熟。LD-FG的优异性能使得缓冲策略成为设计细胞动态成像荧光探针的有效方法。
附：英文原文
Title: Stable super-resolution imaging of lipid droplet dynamics through a buffer strategy with a hydrogen-bond sensitive fluorogenic probe
Author: Jie Chen, Chao Wang, Wenjuan Liu, Qinglong Qiao, Huan Qi, Wei Zhou, Ning Xu, Jin Li, Hailong Piao, Davin Tan, Xiaogang Liu, Zhaochao Xu
Issue&Volume: 2021-09-14
Abstract: Although super-resolution imaging offers an opportunity to visualize cellular structures and organelles at the nanoscale level, cellular heterogeneity and unpredictability still pose a significant challenge in the dynamic imaging of live cells. It is thus vital to develop better performing and more photostable probes for long-term super-resolution imaging. Herein, we report a probe LD-FG for imaging lipid droplet (LD) dynamics using structured illumination microscopy (SIM). LD-FG allows wash-free imaging of LDs, owing to hydrogen-bond sensitive fluorogenicity. The replenishment of photobleached LD-FG by intact ones outside LDs further ensure the long-time stability of the fluorescence imaging. With this buffering fluorogenic probe, fast and unpredictable dynamic processes of LDs can be visualized. Two LD coalescence modes (as well as heterogeneity in different regions of the cells and even in between different cells) were discovered for the first time. Notably, the dynamic imaging also allowed us to propose a new model of LD maturation during adipocyte differentiation, i.e. , a fast LD coalescence followed by a slow ripening step. The excellent performance of LD-FG makes the buffer strategy an effective method for designing fluorescent probes for cell dynamic imaging.
DOI: 10.1002/anie.202111052
Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202111052
