小柯机器人

德国弗莱堡大学Bettina Warscheid、Nils Wiedemann等研究人员合作绘制出定量高可信度的人类线粒体蛋白质组图谱。相关论文于2021年11月19日在线发表于国际学术期刊《细胞—代谢》。

研究人员在人类细胞的线粒体中对8000多个蛋白质进行了分类，并定义了一个由1100多个蛋白质组成的线粒体高置信度蛋白质组（MitoCoP）。研究人员确定了转运酶、呼吸链和ATP合成酶组装因子的相互作用者。MitoCoP蛋白的丰度涵盖了六个数量级，达到了细胞蛋白组的7%，其中伴侣HSP60-HSP10是最丰富的线粒体蛋白。

MitoCoP的动态变化跨越了三个数量级，其半衰期从几小时到几个月不等，这表明生物合成和组装过程的快速调节。460个MitoCoP基因与人类疾病有关，在中枢神经系统和新陈代谢方面有很强的普遍性。MitoCoP将提供一个高置信度的资源，将线粒体的动态、功能和功能障碍置于细胞背景中。

据介绍，线粒体是细胞能量、代谢、信号和质量控制的关键细胞器，与各种疾病有关。关于人类线粒体蛋白质组的组成存在不同的观点。

附：英文原文

Title: Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context

Author: Marcel Morgenstern, Christian D. Peikert, Philipp Lübbert, Ida Suppanz, Cinzia Klemm, Oliver Alka, Conny Steiert, Nataliia Naumenko, Alexander Schendzielorz, Laura Melchionda, Wignand W.D. Mühlhuser, Bettina Knapp, Jakob D. Busch, Sebastian B. Stiller, Stefan Dannenmaier, Caroline Lindau, Mariya Licheva, Christopher Eickhorst, Riccardo Galbusera, Ralf M. Zerbes, Michael T. Ryan, Claudine Kraft, Vera Kozjak-Pavlovic, Friedel Drepper, Sven Dennerlein, Silke Oeljeklaus, Nikolaus Pfanner, Nils Wiedemann, Bettina Warscheid

Issue&Volume: 2021-11-19

Abstract: Mitochondria are key organelles for cellular energetics, metabolism, signaling, and quality control and have been linked to various diseases. Different views exist on the composition of the human mitochondrial proteome. We classified >8,000 proteins in mitochondrial preparations of human cells and defined a mitochondrial high-confidence proteome of >1,100 proteins (MitoCoP). We identified interactors of translocases, respiratory chain, and ATP synthase assembly factors. The abundance of MitoCoP proteins covers six orders of magnitude and amounts to 7% of the cellular proteome with the chaperones HSP60-HSP10 being the most abundant mitochondrial proteins. MitoCoP dynamics spans three orders of magnitudes, with half-lives from hours to months, and suggests a rapid regulation of biosynthesis and assembly processes. 460 MitoCoP genes are linked to human diseases with a strong prevalence for the central nervous system and metabolism. MitoCoP will provide a high-confidence resource for placing dynamics, functions, and dysfunctions of mitochondria into the cellular context.

DOI: 10.1016/j.cmet.2021.11.001

Source: https://www.cell.com/cell-metabolism/fulltext/S1550-4131(21)00529-5

Cell Metabolism：《细胞—代谢》，创刊于2005年。隶属于细胞出版社，最新IF：31.373
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