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One-Pot Synthesis of Multifunctional Carbon-Based Nanoparticle-supported Dispersed Cu2+ Disrupts Redox Homeostasis to Enhance CDT
2021-11-27 22:50

山东师范大学唐波课题组开发出多功能碳基纳米粒子用于增强CDT。相关论文于2021年11月23日在线发表在《德国应用化学》杂志上。

研究人员采用一锅式策略合成了碳基纳米粒子(Cu-cys CBNP),它通过氧化还原反应消耗谷胱甘肽(GSH),产生Cu+,催化H2O2产生-OH,因此通过这种协同效应,活性氧(ROS)水平明显提高。体内实验进一步显示,Cu-cys CBNP可以有效地抑制肿瘤的生长。此外,Cu-cys CBNP可以影响细胞中一些蛋白质巯基的活性,这一点通过rdTOP-ABPP试验进行了评估。

总的来说,这项研究不仅提供了一种潜在的化学动力学治疗(CDT)药物,而且还提供了一种多功能纳米材料的一锅式合成策略。

据悉,在CDT中,ROS的产生水平对评价疗效起着重要作用。然而,肿瘤细胞中高水平的GSH会消耗ROS,直接降低治疗效率。

附:英文原文

Title: One-Pot Synthesis of Multifunctional Carbon-Based Nanoparticle-supported Dispersed Cu2+ Disrupts Redox Homeostasis to Enhance CDT

Author: Jun Lu, Zhongyao Jiang, Jie Ren, Wei Zhang, Ping Li, Zhenzhen Chen, Wen Zhang, Hui Wang, Bo Tang

Issue&Volume: 2021-11-23

Abstract: In chemodynamic therapy (CDT), the levels of reactive oxygen species (ROS) production plays an important role for evaluating the therapeutic efficacy. However, the high levels of glutathione (GSH) in tumor cells consume the ROS, directly reducing the therapeutic efficiency. Herein, we synthesized carbon-based nanoparticle (Cu-cys CBNPs) using one-pot strategy, which consume GSH  via  redox reactions to produce Cu+  that catalyze H2O2  to produce ·OH, thus the ROS level was observably increased through this synergistic effect.  In vivo  experiments further revealed that Cu-cys CBNPs could effectively inhibit tumor growth. Additionally, Cu-cys CBNPs can affect the activity of some protein sulfhydryl groups in cells, which was assessed by rdTOP-ABPP assay. In general, this study not only provides a potential CDT drug, but also provides a strategy for one-pot synthesis of multifunctional nanomaterials.

DOI: 10.1002/anie.202114373

Source: https://onlinelibrary.wiley.com/doi/10.1002/anie.202114373

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