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新方法实现对完整糖肽和修饰聚糖的分析
2021-11-28 13:57

中国科学院计算技术研究所Wen-Feng Zeng等研究人员开发出新方法实现对完整糖肽和修饰聚糖的分析。这一研究成果于2021年11月25日在线发表在国际学术期刊《自然—方法学》上。

研究人员提出了一个聚糖优先的糖肽搜索引擎,pGlyco3,可用于全面分析完整的N-和O-糖肽,包括具有修饰糖单元的糖肽。为聚糖优先搜索而开发的聚糖离子索引算法使pGlyco3比其他糖蛋白组搜索引擎快5-40倍,而不会降低准确性和灵敏度。通过结合基于电子的解离光谱,pGlyco3整合了一个基于动态编程的算法,称为pGlycoSite,可用于特定位点的聚糖定位。

评估表明,由pGlycoSite估计的位点特异性聚糖的定位概率适合定位位点特异性聚糖。通过pGlyco3,研究人员确定了酵母样品中被氨加合物广泛修饰的N-糖肽和O-甘露糖糖肽。免费提供的pGlyco3是一个准确而灵活的工具,可用于鉴定糖肽和修饰的糖单元。

据了解,研究者在完整糖肽分析的质谱数据解释方面已经取得了很大的进展。然而,在特定位点水平和快速准确地识别完整的糖肽和修饰的糖单位仍然是一个挑战。

附:英文原文

Title: Precise, fast and comprehensive analysis of intact glycopeptides and modified glycans with pGlyco3

Author: Zeng, Wen-Feng, Cao, Wei-Qian, Liu, Ming-Qi, He, Si-Min, Yang, Peng-Yuan

Issue&Volume: 2021-11-25

Abstract: Great advances have been made in mass spectrometric data interpretation for intact glycopeptide analysis. However, accurate identification of intact glycopeptides and modified saccharide units at the site-specific level and with fast speed remains challenging. Here, we present a glycan-first glycopeptide search engine, pGlyco3, to comprehensively analyze intact N- and O-glycopeptides, including glycopeptides with modified saccharide units. A glycan ion-indexing algorithm developed for glycan-first search makes pGlyco3 5–40 times faster than other glycoproteomic search engines without decreasing accuracy or sensitivity. By combining electron-based dissociation spectra, pGlyco3 integrates a dynamic programming-based algorithm termed pGlycoSite for site-specific glycan localization. Our evaluation shows that the site-specific glycan localization probabilities estimated by pGlycoSite are suitable to localize site-specific glycans. With pGlyco3, we confidently identified N-glycopeptides and O-mannose glycopeptides that were extensively modified by ammonia adducts in yeast samples. The freely available pGlyco3 is an accurate and flexible tool that can be used to identify glycopeptides and modified saccharide units.

DOI: 10.1038/s41592-021-01306-0

Source: https://www.nature.com/articles/s41592-021-01306-0

Nature Methods:《自然—方法学》,创刊于2004年。隶属于施普林格·自然出版集团,最新IF:47.99
官方网址:https://www.nature.com/nmeth/
投稿链接:https://mts-nmeth.nature.com/cgi-bin/main.plex


本期文章:《自然—方法学》:Online/在线发表

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