小柯机器人

瑞士苏黎世联邦理工学院 Tommaso Patriarchi小组取得一项新突破。他们的最新研究研发了一种用于食欲素神经肽体内成像的基因编码传感器。这一研究成果于2022年2月10日发表在国际顶尖学术期刊《自然—方法学》上。

在本研究中，研究人员研发了一种基因编码的食欲素传感器 (OxLight1)，该传感器将循环排列的绿色荧光蛋白工程改造为人类2型食欲素受体。在小鼠中，OxLight1能以高灵敏度方式在体内检测光遗传学诱导的内源性食欲素释放。小鼠体内OxLight1的光度记录显示，食欲素的快速释放与不同大脑区域调控的跑步行为、急性压力和睡眠到觉醒的转变有关。

此外，OxLight1双光子成像揭示了麻醉苏醒期间小鼠体感皮层2/3层的食欲素释放。因此，OxLight1能够在活体动物中以高时空分辨率对食欲素神经肽进行直接且灵敏的光学检测。

据悉，食欲素（也称下丘脑分泌素）是中枢神经系统中发挥重要功能的下丘脑神经肽；然而，由于当前检测技术分辨率有限，对其在体内的释放和作用范围知之甚少。

附：英文原文

Title: A genetically encoded sensor for in vivo imaging of orexin neuropeptides

Author: Duffet, Loc, Kosar, Seher, Panniello, Mariangela, Viberti, Bianca, Bracey, Edward, Zych, Anna D., Radoux-Mergault, Arthur, Zhou, Xuehan, Dernic, Jan, Ravotto, Luca, Tsai, Yuan-Chen, Figueiredo, Marta, Tyagarajan, Shiva K., Weber, Bruno, Stoeber, Miriam, Gogolla, Nadine, Schmidt, Markus H., Adamantidis, Antoine R., Fellin, Tommaso, Burdakov, Denis, Patriarchi, Tommaso

Issue&Volume: 2022-02-10

Abstract: Orexins (also called hypocretins) are hypothalamic neuropeptides that carry out essential functions in the central nervous system; however, little is known about their release and range of action in vivo owing to the limited resolution of current detection technologies. Here we developed a genetically encoded orexin sensor (OxLight1) based on the engineering of circularly permutated green fluorescent protein into the human type-2 orexin receptor. In mice OxLight1 detects optogenetically evoked release of endogenous orexins in vivo with high sensitivity. Photometry recordings of OxLight1 in mice show rapid orexin release associated with spontaneous running behavior, acute stress and sleep-to-wake transitions in different brain areas. Moreover, two-photon imaging of OxLight1 reveals orexin release in layer 2/3 of the mouse somatosensory cortex during emergence from anesthesia. Thus, OxLight1 enables sensitive and direct optical detection of orexin neuropeptides with high spatiotemporal resolution in living animals. OxLight1 is a genetically encoded sensor for the orexin neuropeptides. It has been applied in fiber photometry recordings and two-photon imaging in mice during a variety of behaviors.

DOI: 10.1038/s41592-021-01390-2

Source: https://www.nature.com/articles/s41592-021-01390-2

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex