美国国家癌症研究所Martin J. Schnermann和德国亥姆霍兹慕尼黑研究中心Oliver T. Bruns小组合作在研究中取得进展。他们发现九甲胺花青可用于超过1000纳米的多色靶向体内成像。2022年2月28日，国际学术期刊《自然-方法学》发表了这一成果。
通过合理的设计，研究人员分别通过邻苯二酚环和芳基环融合在壬二胺骨架上合成了最大吸光度为872和1072nm的过磺化吲哚菁染料。在几种肿瘤模型中使用单克隆抗体和葡聚糖偶联物进行的多路双色和三色体内成像表明，该染料可同时标记肿瘤和健康周围组织和淋巴管。这些发现得益于定制的近红外/短波红外成像设置和用于多色实时成像软件包的更新。
据了解，最近的研究表明，使用1000和2000nm之间的波长（短波红外或近红外(NIR)-II范围内）可实现传统光学波长无法进行的高分辨率体内成像。已经证明可见光和NIR范围内多路复用可用于成像生物，然而这种共轭探针在成像领域的应用还很稀少。
附：英文原文
Title: Targeted multicolor in vivo imaging over 1,000 nm enabled by nonamethine cyanines
Author: Bandi, Venu G., Luciano, Michael P., Saccomano, Mara, Patel, Nimit L., Bischof, Thomas S., Lingg, Jakob G. P., Tsrunchev, Peter T., Nix, Meredith N., Ruehle, Bastian, Sanders, Chelsea, Riffle, Lisa, Robinson, Christina M., Difilippantonio, Simone, Kalen, Joseph D., Resch-Genger, Ute, Ivanic, Joseph, Bruns, Oliver T., Schnermann, Martin J.
Issue&Volume: 2022-02-28
Abstract: Recent progress has shown that using wavelengths between 1,000 and 2,000nm, referred to as the shortwave-infrared or near-infrared (NIR)-II range, can enable high-resolution in vivo imaging at depths not possible with conventional optical wavelengths. However, few bioconjugatable probes of the type that have proven invaluable for multiplexed imaging in the visible and NIR range are available for imaging these wavelengths. Using rational design, we have generated persulfonated indocyanine dyes with absorbance maxima at 872 and 1,072nm through catechol-ring and aryl-ring fusion, respectively, onto the nonamethine scaffold. Multiplexed two-color and three-color in vivo imaging using monoclonal antibody and dextran conjugates in several tumor models illustrate the benefits of concurrent labeling of the tumor and healthy surrounding tissue and lymphatics. These efforts are enabled by complementary advances in a custom-built NIR/shortwave-infrared imaging setup and software package for multicolor real-time imaging.
DOI: 10.1038/s41592-022-01394-6
Source: https://www.nature.com/articles/s41592-022-01394-6
Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex
本期文章：《自然—方法学》：Online/在线发表
