小柯机器人

日本大阪大学医学院Ryuhei Hayashi团队近期取得重要工作进展，他们利用人类多能干细胞生成三维泪腺类器官。这一成果2022年4月20日在线发表于《自然》杂志上。

研究人员识别出具有泪腺原基特征的细胞，这些细胞出现在从人类多能干细胞培养的二维眼状眼类器官中。当通过细胞分选分离并在特定条件下生长时，细胞形成具有导管和腺泡的三维泪腺样组织器官，通过出芽和分枝实现。克隆集落分析表明类器官来源于多能眼表上皮干细胞。这些类器官在形态、免疫标记特征和基因表达的基础模式上表现出与天然泪腺的显著相似之处，并且在移植到受体大鼠的眼睛附近时经历功能成熟，形成腔并产生泪膜蛋白。

据介绍，泪腺是眼睛的主要外分泌腺。它们位于眼眶内，在上眼睑后面，朝向每只眼睛的颞侧，分泌泪液，作为泪膜的主要成分。

附：英文原文

Title: Generation of 3D lacrimal gland organoids from human pluripotent stem cells

Author: Hayashi, Ryuhei, Okubo, Toru, Kudo, Yuji, Ishikawa, Yuki, Imaizumi, Tsutomu, Suzuki, Kenji, Shibata, Shun, Katayama, Tomohiko, Park, Sung-Joon, Young, Robert D., Quantock, Andrew J., Nishida, Kohji

Issue&Volume: 2022-04-20

Abstract: Lacrimal glands are the main exocrine glands of the eyes. Situated within the orbit, behind the upper eyelid and towards the temporal side of each eye, they secrete lacrimal fluid as a major component of the tear film. Here we identify cells with characteristics of lacrimal gland primordia that emerge in two-dimensional eye-like organoids cultured from human pluripotent stem cells1. When isolated by cell sorting and grown under defined conditions, the cells form a three-dimensional lacrimal-gland-like tissue organoid with ducts and acini, enabled by budding and branching. Clonal colony analyses indicate that the organoids originate from multipotent ocular surface epithelial stem cells. The organoids exhibit notable similarities to native lacrimal glands on the basis of their morphology, immunolabelling characteristics and gene expression patterns, and undergo functional maturation when transplanted adjacent to the eyes of recipient rats, developing lumina and producing tear-film proteins. Organoids originating from human multipotent ocular surface epithelial stem cells are similar to native lacrimal glands and undergo functional maturation when transplanted adjacent to the eyes of recipient rats, developing lumina and producing tear-film proteins.

DOI: 10.1038/s41586-022-04613-4

Source: https://www.nature.com/articles/s41586-022-04613-4

Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
官方网址：http://www.nature.com/
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