小柯机器人

2022年6月16日，《自然—方法学》杂志在线发表了德国科学家的一项最新研究成果。来自慕尼黑工业大学的Bernhard Kuster课题组绘制出基于质谱技术的小鼠蛋白质组图谱。

研究人员提出了一个由41个健康组织构建的小鼠蛋白质组和磷蛋白组的定量图谱，并有几条分析线例证了可以从数据中收集到哪些见解。例如，组织和细胞类型的解析图谱为体内17,000个基因、数千个亚型和50,000个磷酸化位点的表达提供了蛋白质证据。小鼠、人类和拟南芥的蛋白质基因组比较揭示了基因表达调控的共同和不同的机制，尽管有许多相似之处，但有许多不同的丰富的同系物，可能服务于特定物种的功能。

研究人员通过整合表型药物（n>400）和辐射反应数据与66个胰腺导管腺癌（PDAC）细胞系的蛋白质组来揭示了敏感性和抗性的分子标记。这个独特的图谱补充了其他小鼠的分子资源，可以通过ProteomicsDB和PACiFIC在线搜索。

据了解，实验小鼠是生物医学研究中最重要的实验系统之一，这种模型的分子参考图是必不可少的信息工具。

附：英文原文

Title: Mass spectrometry-based draft of the mouse proteome

Author: Giansanti, Piero, Samaras, Patroklos, Bian, Yangyang, Meng, Chen, Coluccio, Andrea, Frejno, Martin, Jakubowsky, Hannah, Dobiasch, Sophie, Hazarika, Rashmi R., Rechenberger, Julia, Calzada-Wack, Julia, Krumm, Johannes, Mueller, Sebastian, Lee, Chien-Yun, Wimberger, Nicole, Lautenbacher, Ludwig, Hassan, Zonera, Chang, Yun-Chien, Falcomat, Chiara, Bayer, Florian P., Brthel, Stefanie, Schmidt, Tobias, Rad, Roland, Combs, Stephanie E., The, Matthew, Johannes, Frank, Saur, Dieter, de Angelis, Martin Hrabe, Wilhelm, Mathias, Schneider, Gnter, Kuster, Bernhard

Issue&Volume: 2022-06-16

Abstract: The laboratory mouse ranks among the most important experimental systems for biomedical research and molecular reference maps of such models are essential informational tools. Here, we present a quantitative draft of the mouse proteome and phosphoproteome constructed from 41 healthy tissues and several lines of analyses exemplify which insights can be gleaned from the data. For instance, tissue- and cell-type resolved profiles provide protein evidence for the expression of 17,000 genes, thousands of isoforms and 50,000 phosphorylation sites in vivo. Proteogenomic comparison of mouse, human and Arabidopsis reveal common and distinct mechanisms of gene expression regulation and, despite many similarities, numerous differentially abundant orthologs that likely serve species-specific functions. We leverage the mouse proteome by integrating phenotypic drug (n>400) and radiation response data with the proteomes of 66 pancreatic ductal adenocarcinoma (PDAC) cell lines to reveal molecular markers for sensitivity and resistance. This unique atlas complements other molecular resources for the mouse and can be explored online via ProteomicsDB and PACiFIC.

DOI: 10.1038/s41592-022-01526-y

Source: https://www.nature.com/articles/s41592-022-01526-y

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex