小柯机器人

荷兰马斯特里赫特大学Pieter Goossens等研究人员通过整合多重免疫荧光和质谱成像技术，在代谢和细胞背景下绘制出骨髓异质性图谱。该项研究成果于2022年7月19日在线发表在《细胞—代谢》杂志上。

研究人员提出了一种新的综合方法，其中15色多光谱成像允许基于多标记物表达模式的综合细胞分类，然后通过下游分析管道将其表型与背景、微环境线索联系起来，如复杂组织中的细胞（"社区"）和代谢（"局部脂质组"）微环境。这种方法的威力在小鼠动脉粥样硬化斑块的骨髓亚群和相关脂质特征中得到了说明。

据介绍，细胞经常采取不同的表型，由组织特异性或局部信号决定，如细胞-细胞和细胞-基质接触或分子微环境。这对具有高度表型可塑性的巨噬细胞来说是极端的。它们广泛的功能，有些甚至是对立的，反映了它们的异质性，在不同的组织和疾病中描述了众多的亚群。这种微环境印记不能通过单细胞应用来充分研究，因为细胞脱离了它们的环境，而基于组织学的评估由于标志物组合的限制而缺乏表型深度。

附：英文原文

Title: Integrating multiplex immunofluorescent and mass spectrometry imaging to map myeloid heterogeneity in its metabolic and cellular context

Author: Pieter Goossens, Chang Lu, Jianhua Cao, Marion J. Gijbels, Jol M.H. Karel, Erwin Wijnands, Britt S.R. Claes, Gregorio E. Fazzi, Tim F.E. Hendriks, Kristiaan Wouters, Evgueni Smirnov, Marc J.M. van Zandvoort, Benjamin Balluff, Eva Cuypers, Marjo M.P.C. Donners, Ron M.A. Heeren, Erik A.L. Biessen

Issue&Volume: 2022-07-19

Abstract: Cells often adopt different phenotypes, dictated by tissue-specific or local signals such as cell-cell and cell-matrix contacts or molecular micro-environment. This holds in extremis for macrophages with their high phenotypic plasticity. Their broad range of functions, some even opposing, reflects their heterogeneity, and a multitude of subsets has been described in different tissues and diseases. Such micro-environmental imprint cannot be adequately studied by single-cell applications, as cells are detached from their context, while histology-based assessment lacks the phenotypic depth due to limitations in marker combination. Here, we present a novel, integrative approach in which 15-color multispectral imaging allows comprehensive cell classification based on multi-marker expression patterns, followed by downstream analysis pipelines to link their phenotypes to contextual, micro-environmental cues, such as their cellular (“community”) and metabolic (“local lipidome”) niches in complex tissue. The power of this approach is illustrated for myeloid subsets and associated lipid signatures in murine atherosclerotic plaque.

DOI: 10.1016/j.cmet.2022.06.012

Source: https://www.cell.com/cell-metabolism/fulltext/S1550-4131(22)00232-7

Cell Metabolism：《细胞—代谢》，创刊于2005年。隶属于细胞出版社，最新IF：31.373
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