小柯机器人

美国莱斯大学Franois St-Pierre研究小组使用快速指示器演变的双光子显微镜实现持续的深层组织电压记录。这一研究成果于2022年8月18日在线发表在国际学术期刊《细胞》上。

研究人员表示，基因编码的电压指示器是监测具有细胞类型特异性的电压动态的新兴工具。然而，目前的指示器由于在双光子显微镜下的性能不佳，使其应用范围狭窄，而双光子显微镜是深层组织记录的首选方法。

为了改善指示器，研究人员开发了一个多参数高通量平台，以优化双光子显微镜的电压指示器。利用这个系统，研究人员确定了JEDI-2P，一个比其前任更快、更亮、更敏感和光稳定的指示器。研究人员证明JEDI-2P可以报告果蝇中间神经元的轴突末端和分离的小鼠视网膜的树突及杏仁核细胞的光诱导反应。JEDI-2P还可以使用共振扫描和ULoVE随机访问显微镜，在清醒的行为小鼠中光学记录单个皮质神经元的电压动态超过30分钟。最后，JEDI-2P的ULoVE记录可以在超过400微米的深度有力地检测脉冲，并报告神经元对的电压相关性。

附：英文原文

Title: Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy

Author: Zhuohe Liu, Xiaoyu Lu, Vincent Villette, Yueyang Gou, Kevin L. Colbert, Shujuan Lai, Sihui Guan, Michelle A. Land, Jihwan Lee, Tensae Assefa, Daniel R. Zollinger, Maria M. Korympidou, Anna L. Vlasits, Michelle M. Pang, Sharon Su, Changjia Cai, Emmanouil Froudarakis, Na Zhou, Saumil S. Patel, Cameron L. Smith, Annick Ayon, Pierre Bizouard, Jonathan Bradley, Katrin Franke, Thomas R. Clandinin, Andrea Giovannucci, Andreas S. Tolias, Jacob Reimer, Stéphane Dieudonné, Franois St-Pierre

Issue&Volume: 2022-08-18

Abstract: Genetically encoded voltage indicators are emerging tools for monitoring voltage dynamics with cell-type specificity. However, current indicators enable a narrow range of applications due to poor performance under two-photon microscopy, a method of choice for deep-tissue recording. To improve indicators, we developed a multiparameter high-throughput platform to optimize voltage indicators for two-photon microscopy. Using this system, we identified JEDI-2P, an indicator that is faster, brighter, and more sensitive and photostable than its predecessors. We demonstrate that JEDI-2P can report light-evoked responses in axonal termini of Drosophila interneurons and the dendrites and somata of amacrine cells of isolated mouse retina. JEDI-2P can also optically record the voltage dynamics of individual cortical neurons in awake behaving mice for more than 30 min using both resonant-scanning and ULoVE random-access microscopy. Finally, ULoVE recording of JEDI-2P can robustly detect spikes at depths exceeding 400 μm and report voltage correlations in pairs of neurons.

DOI: 10.1016/j.cell.2022.07.013

Source: https://www.cell.com/cell/fulltext/S0092-8674(22)00916-3