小柯机器人

瑞典KTH皇家理工学院Ilaria Testa研究团队取得一项新突破。他们研发了事件触发的STED成像。这一研究成果于2022年9月8日发表在国际学术期刊《自然-方法学》上。

研究人员研发了事件触发STED，这是一种自动化的多尺度方法，能够在用生物传感器检测到蛋白质募集、囊泡运输和第二信使等细胞事件后快速启动二维(2D)和3D STED成像。STED具有检测事件内的最大时间分辨率。研究人员在高达24 Hz、40 ms的局部钙活动后对突触小泡动力学进行成像；在局部蛋白质招募或pH值变化后，以高达11 Hz、40 ms的速率检测到内吞和外吞事件；以及在3 Hz、70 ms的分辨率检测到内体囊泡之间的相互作用和彼此接近。事件触发STED扩展了实时纳米级成像的能力，能够对新生物进行实时观察。

据了解，探究参与所有细胞过程的蛋白质和脂质需要具有更高空间和时间分辨率的成像方法。STED（受激发射损耗）纳米镜能够对活细胞中的纳米级结构进行快速成像，但受到光漂白的限制。

附：英文原文

Title: Event-triggered STED imaging

Author: Alvelid, Jonatan, Damenti, Martina, Sgattoni, Chiara, Testa, Ilaria

Issue&Volume: 2022-09-08

Abstract: Monitoring the proteins and lipids that mediate all cellular processes requires imaging methods with increased spatial and temporal resolution. STED (stimulated emission depletion) nanoscopy enables fast imaging of nanoscale structures in living cells but is limited by photobleaching. Here, we present event-triggered STED, an automated multiscale method capable of rapidly initiating two-dimensional (2D) and 3D STED imaging after detecting cellular events such as protein recruitment, vesicle trafficking and second messengers activity using biosensors. STED is applied in the vicinity of detected events to maximize the temporal resolution. We imaged synaptic vesicle dynamics at up to 24Hz, 40ms after local calcium activity; endocytosis and exocytosis events at up to 11Hz, 40ms after local protein recruitment or pH changes; and the interaction between endosomal vesicles at up to 3Hz, 70ms after approaching one another. Event-triggered STED extends the capabilities of live nanoscale imaging, enabling novel biological observations in real time.

DOI: 10.1038/s41592-022-01588-y

Source: https://www.nature.com/articles/s41592-022-01588-y

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
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