小柯机器人

日本大阪大学Daisuke Ino研究组制造用于实时测量大脑细胞外催产素（OT）动力学的荧光传感器。相关论文发表在2022年9月22日出版的《自然—方法学》杂志上。

他们描述了MTRIAOT，这是一种基于G蛋白偶联受体的绿色荧光OT传感器，该传感器具有较大的动态范围，合适的亲和力，对OT直系同源物的配体特异性，对下游信号传导和长期荧光稳定性的最小影响。通过结合病毒基因递送和光纤光度法介导的荧光测定，他们证明了Mtriaot对活小鼠中脑OT动力学实时检测的实用性。MTRIAOT介导的测定表明，OT动力学的变异性依赖于动物的行为环境和物理状况。MTRIAOT可能可以在多种生理和病理过程中对OT动力学进行分析。

研究人员表示，OT是一种在大脑中充当神经调节剂的下丘脑神经肽，它协调了多种动物行为。但是，大脑OT动力学与复杂动物行为之间的关系仍然未知，部分原因是缺乏适合其在体内实时记录的技术。

附：英文原文

Title: A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain

Author: Ino, Daisuke, Tanaka, Yudai, Hibino, Hiroshi, Nishiyama, Masaaki

Issue&Volume: 2022-09-22

Abstract: Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo. Here, we describe MTRIAOT, a G-protein-coupled receptor-based green fluorescent OT sensor that has a large dynamic range, suitable affinity, ligand specificity for OT orthologs, minimal effects on downstream signaling and long-term fluorescence stability. By combining viral gene delivery and fiber photometry-mediated fluorescence measurements, we demonstrate the utility of MTRIAOT for real-time detection of brain OT dynamics in living mice. MTRIAOT-mediated measurements indicate variability of OT dynamics depending on the behavioral context and physical condition of an animal. MTRIAOT will likely enable the analysis of OT dynamics in a variety of physiological and pathological processes.

DOI: 10.1038/s41592-022-01597-x

Source: https://www.nature.com/articles/s41592-022-01597-x

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex