小柯机器人

比利时列日大学Thomas Marichal研究组的研究表明，mafb限制性局部单核细胞增殖会优先于肺间质巨噬细胞分化。这一研究成果于2023年3月16日发表在国际学术期刊《自然—免疫学》上。

研究人员使用诱导肺间质巨噬细胞（IM）生态位耗竭和再移植小鼠模型来研究体内IM的发展。使用时程单细胞RNA测序分析、骨髓嵌合体和基因靶向，研究发现移植的典型的Ly6C⁺单核细胞在分化为IM之前以Csf1受体依赖性的方式局部增殖。转录因子MafB调控了从单核细胞增殖到IM亚群的转变，而c-Maf特异性调控CD206⁺ IM亚群的特征。

该研究提供的证据表明在单核吞噬细胞系统中，增殖能力不仅限于髓系祖细胞和成熟的驻留组织巨噬细胞（RTM），而且在体内单核细胞发育成RTMs的能力也受到严格调控。

据介绍，RTM是分化的免疫细胞，它们分布于不同的生态位并发挥重要的组织支持功能。RTM的维持依赖于单核细胞分化或RTM自我更新。

附：英文原文

Title: MafB-restricted local monocyte proliferation precedes lung interstitial macrophage differentiation

Author: Vanneste, Domien, Bai, Qiang, Hasan, Shakir, Peng, Wen, Pirottin, Dimitri, Schyns, Joey, Marchal, Pauline, Ruscitti, Cecilia, Meunier, Margot, Liu, Zhaoyuan, Legrand, Cline, Fievez, Laurence, Ginhoux, Florent, Radermecker, Coraline, Bureau, Fabrice, Marichal, Thomas

Issue&Volume: 2023-03-16

Abstract: Resident tissue macrophages (RTMs) are differentiated immune cells that populate distinct niches and exert important tissue-supportive functions. RTM maintenance is thought to rely either on differentiation from monocytes or on RTM self-renewal. Here, we used a mouse model of inducible lung interstitial macrophage (IM) niche depletion and refilling to investigate the development of IMs in vivo. Using time-course single-cell RNA-sequencing analyses, bone marrow chimeras and gene targeting, we found that engrafted Ly6C+ classical monocytes proliferated locally in a Csf1 receptor-dependent manner before differentiating into IMs. The transition from monocyte proliferation toward IM subset specification was controlled by the transcription factor MafB, while c-Maf specifically regulated the identity of the CD206+ IM subset. Our data provide evidence that, in the mononuclear phagocyte system, the ability to proliferate is not merely restricted to myeloid progenitor cells and mature RTMs but is also a tightly regulated capability of monocytes developing into RTMs in vivo.

DOI: 10.1038/s41590-023-01468-3

Source: https://www.nature.com/articles/s41590-023-01468-3

Nature Immunology：《自然—免疫学》，创刊于2000年。隶属于施普林格·自然出版集团，最新IF：31.25
官方网址：https://www.nature.com/ni/
投稿链接：https://mts-ni.nature.com/cgi-bin/main.plex