小柯机器人

中国海洋大学施威扬和美国纪念斯隆凯瑟琳癌症研究所鲍志戎研究团队合作绘制了人类胚胎早期器官发生的单细胞转录组图谱。该研究于2023年3月16日发表于国际一流学术期刊《自然—细胞生物学》杂志上。

他们研究了4-6周的人类胚胎的细胞多样性，当时基本上所有器官都刚刚分布好。在超过180,000个单细胞转录组的基础上，他们生成了18个发育系统中313个簇的综合图谱，并使用来自157份出版物的本体和标记进行了注释。结合胚胎切片上的空间转录组，他们描述了以前未被重视的细胞类型的分子和空间结构。结合其他脊椎动物的数据，这些丰富的信息揭示了轴的空间模式、发育进程的系统性时间调节和潜在的人类特异性调节。

本研究提供了人类器官早期祖细胞的概况，可作为器官发生谱系分析的基础。

附：英文原文

Title: A single-cell transcriptome atlas profiles early organogenesis in human embryos

Author: Xu, Yichi, Zhang, Tengjiao, Zhou, Qin, Hu, Mengzhu, Qi, Yao, Xue, Yifang, Nie, Yuxiao, Wang, Lihui, Bao, Zhirong, Shi, Weiyang

Issue&Volume: 2023-03-16

Abstract: The early window of human embryogenesis is largely a black box for developmental biologists. Here we probed the cellular diversity of 4–6week human embryos when essentially all organs are just laid out. On the basis of over 180,000 single-cell transcriptomes, we generated a comprehensive atlas of 313 clusters in 18 developmental systems, which were annotated with a collection of ontology and markers from 157 publications. Together with spatial transcriptome on embryonic sections, we characterized the molecule and spatial architecture of previously unappreciated cell types. Combined with data from other vertebrates, the rich information shed light on spatial patterning of axes, systemic temporal regulation of developmental progression and potential human-specific regulation. Our study provides a compendium of early progenitor cells of human organs, which can serve as the root of lineage analysis in organogenesis.

DOI: 10.1038/s41556-023-01108-w

Source: https://www.nature.com/articles/s41556-023-01108-w

Nature Cell Biology：《自然—细胞生物学》，创刊于1999年。隶属于施普林格·自然出版集团，最新IF：28.213
官方网址：https://www.nature.com/ncb/
投稿链接：https://mts-ncb.nature.com/cgi-bin/main.plex