小柯机器人

厦门大学Jiahuai Han和Xiurong Wu共同合作，近期取得重要工作进展。他们的最新研究发现，核糖体拯救者PELO通过激活NOD样受体家族蛋白的ATP酶活性来催化其寡聚组装。相关研究成果2023年3月21日在线发表于《免疫学》杂志上。

据介绍，NOD样受体（NLR）是多种先天免疫反应的模式识别受体。与配体结合后的自寡聚化是每种NLR活化的普遍接受的模型。

研究人员报道了一种NLR自寡聚化需要催化剂。PELO是翻译质量控制及核糖体拯救中众所周知的监测因子，它与所有胞质NLR相互作用并激活其ATP酶活性。在鞭毛蛋白引发NLRC4炎症小体激活的情况下，鞭毛蛋白结合的NAIP5募集第一个NLRC4，然后需要PELO通过激活NLRC4 ATP酶活性，将其余的NLRC4一个接一个地正确组装到NLRC4复合物中。化学计量和功能数据显示，PELO不是NLRC4炎症小体的结构成分，而是其组装的强大催化剂。

总之，PELO在胞质NLR活化中的催化作用为NLR活化提供了见解，并为NLR家族成员的未来研究提供了方向。

附：英文原文

Title: Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity

Author: Xiurong Wu, Zhang-Hua Yang, Jianfeng Wu, Jiahuai Han

Issue&Volume: 2023-03-21

Abstract: NOD-like receptors (NLRs) are pattern recognition receptors for diverse innate immuneresponses. Self-oligomerization after engagement with a ligand is a generally acceptedmodel for the activation of each NLR. We report here that a catalyzer was requiredfor NLR self-oligomerization. PELO, a well-known surveillance factor in translationalquality control and/or ribosome rescue, interacted with all cytosolic NLRs and activatedtheir ATPase activity. In the case of flagellin-initiated NLRC4 inflammasome activation,flagellin-bound NAIP5 recruited the first NLRC4 and then PELO was required for correctlyassembling the rest of NLRC4s into the NLRC4 complex, one by one, by activating theNLRC4 ATPase activity. Stoichiometric and functional data revealed that PELO was nota structural constituent of the NLRC4 inflammasome but a powerful catalyzer for itsassembly. The catalytic role of PELO in the activation of cytosolic NLRs providesinsight into NLR activation and provides a direction for future studies of NLR familymembers.

DOI: 10.1016/j.immuni.2023.02.014

Source: https://www.cell.com/immunity/fulltext/S1074-7613(23)00088-2

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