小柯机器人

美国斯坦福大学Sergiu P. Paca研究团队的最新研究绘制了发育中人脊髓的单细胞转录组图谱。该项研究成果发表在2023年4月24日出版的《自然—神经科学》上。

研究人员利用单细胞分辨率转录组学分析了孕期人脊髓的发育过程，并发现了细胞类型之间和细胞类型内的显著异质性。神经胶质细胞沿背腹轴和前尾轴显示出与位置同一性相关的多样性，而具有特殊转录程序的星形胶质细胞映射到白质和灰质亚型。运动神经元在这个阶段聚集成α和γ神经元组。研究人员还将获得的数据与人脊髓跨越22周妊娠发育的多个现有数据集相结合，以研究细胞多样性随时间的变化。

与疾病相关基因映射一起，发育中人类脊髓转录组图谱为揭示在细胞水平如何调控人类运动开辟了新的途径，并有利于基于人类干细胞的疾病模型建立。

据悉，了解脊髓组装对于阐明如何控制运动行为以及疾病如何产生至关重要。人体脊髓组织很精密，这种复杂的组织有助于运动和感觉处理的多样性和复杂性。但是在人类脊髓细胞水平上如何产生这种复杂性仍然未知。

附：英文原文

Title: Single-cell transcriptomic landscape of the developing human spinal cord

Author: Andersen, Jimena, Thom, Nicholas, Shadrach, Jennifer L., Chen, Xiaoyu, Onesto, Massimo Mario, Amin, Neal D., Yoon, Se-Jin, Li, Li, Greenleaf, William J., Mller, Fabian, Paca, Anca M., Kaltschmidt, Julia A., Paca, Sergiu P.

Issue&Volume: 2023-04-24

Abstract: Understanding spinal cord assembly is essential to elucidate how motor behavior is controlled and how disorders arise. The human spinal cord is exquisitely organized, and this complex organization contributes to the diversity and intricacy of motor behavior and sensory processing. But how this complexity arises at the cellular level in the human spinal cord remains unknown. Here we transcriptomically profiled the midgestation human spinal cord with single-cell resolution and discovered remarkable heterogeneity across and within cell types. Glia displayed diversity related to positional identity along the dorso-ventral and rostro-caudal axes, while astrocytes with specialized transcriptional programs mapped into white and gray matter subtypes. Motor neurons clustered at this stage into groups suggestive of alpha and gamma neurons. We also integrated our data with multiple existing datasets of the developing human spinal cord spanning 22weeks of gestation to investigate the cell diversity over time. Together with mapping of disease-related genes, this transcriptomic mapping of the developing human spinal cord opens new avenues for interrogating the cellular basis of motor control in humans and guides human stem cell-based models of disease.

DOI: 10.1038/s41593-023-01311-w

Source: https://www.nature.com/articles/s41593-023-01311-w

Nature Neuroscience：《自然—神经科学》，创刊于1998年。隶属于施普林格·自然出版集团，最新IF：28.771
官方网址：https://www.nature.com/neuro/
投稿链接：https://mts-nn.nature.com/cgi-bin/main.plex