小柯机器人

澳大利亚墨尔本大学Laura K. Mackay和Maximilien Evrard共同合作，近期取得重要工作进展。他们通过研究单细胞蛋白表达谱解析跨组织和感染环境的中循环和常驻记忆T细胞的多样性。相关研究成果2023年6月30日在线发表于《免疫》杂志上。

据介绍，记忆性CD8⁺ T细胞可广泛分为循环（T_CIRCM）和组织驻留记忆性T（T_RM）细胞群体。尽管存在明确的迁移和转录差异，但T_CIRCM和T_RM细胞的表型和功能特征，特别是跨组织的表型和职能特征，仍然难以捉摸。

研究人员利用抗体筛选平台和机器学习预测管道(InfinityFlow)分析了实体器官和屏障位置的T_CIRCM和T_RM细胞中的200多种蛋白质。高维分析显示，在局部或全身小鼠感染模型后，九个不同器官的T_CIRCM和T_RM细胞系中存在未被识别的异质性。

此外，研究人员证明了允许跨器官选择性消融T_CIRCM或T_RM群体策略的相对有效性，并确定CD55、KLRG1、CXCR6和CD38是表征炎症期间记忆T细胞功能的稳定标志物。

总之，这些数据和分析框架为稳态和炎症条件下的记忆T细胞分类提供了深入的资源。

附：英文原文

Title: Single-cell protein expression profiling resolves circulating and resident memory T cell diversity across tissues and infection contexts

Author: Maximilien Evrard, Etienne Becht, Raissa Fonseca, Andreas Obers, Simone L. Park, Nagela Ghabdan-Zanluqui, Jan Schroeder, Susan N. Christo, Dominik Schienstock, Junyun Lai, Thomas N. Burn, Allison Clatch, Imran G. House, Paul Beavis, Axel Kallies, Florent Ginhoux, Scott N. Mueller, Raphael Gottardo, Evan W. Newell, Laura K. Mackay

Issue&Volume: 2023-06-30

Abstract: Memory CD8+ T cells can be broadly divided into circulating (TCIRCM) and tissue-resident memory T (TRM) populations. Despite well-defined migratory and transcriptional differences, thephenotypic and functional delineation of TCIRCM and TRM cells, particularly across tissues, remains elusive. Here, we utilized an antibodyscreening platform and machine learning prediction pipeline (InfinityFlow) to profile>200 proteins in TCIRCM and TRM cells in solid organs and barrier locations. High-dimensional analyses revealed unappreciatedheterogeneity within TCIRCM and TRM cell lineages across nine different organs after either local or systemic murineinfection models. Additionally, we demonstrated the relative effectiveness of strategiesallowing for the selective ablation of TCIRCM or TRM populations across organs and identified CD55, KLRG1, CXCR6, and CD38 as stable markersfor characterizing memory T cell function during inflammation. Together, these dataand analytical framework provide an in-depth resource for memory T cell classificationin both steady-state and inflammatory conditions.

DOI: 10.1016/j.immuni.2023.06.005

Source: https://www.cell.com/immunity/fulltext/S1074-7613(23)00262-5

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