德国维尔茨堡大学Wolfgang Kastenmüller课题组发现，淋巴结(LNs)髓质调节树突状细胞(cDC)网络的时空展开。2023年7月17日，国际知名学术期刊《免疫》发表了这一成果。
他们检验了是否存在特定的解剖壁龛，其中DC前体(preDCs)向未成熟的cDC分化。原位光转化和基于Prtn3的命运追踪显示，LN髓索是preDCs的优先进入位点，作为特异性分化生态位。再生和命运追踪方法表明，cDC1网络从髓质沿着血管树向副皮层展开。在炎症期间，常驻cDC1的集体成熟和迁移到皮层旁，造成了髓质cDC1网络的不连续性，暂时减弱了反应性。局部cDC1密度的降低导致髓质生态位中Flt3L可用性升高，从而加速了cDC1的发展以恢复网络。因此，cDC1网络的时空发展是通过感知cDC1密度在专用的LN生态位中进行局部调节的。
据悉，与巨噬细胞网络由特定壁龛内的长寿命组织驻留细胞组成不同，在LN中产生3D网络的传统cDC寿命较短，并且不断被来自骨髓(BM)的preDC所取代。
附：英文原文
Title: Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networks
Author: Milas Ugur, R. Jacob Labios, Chloe Fenton, Konrad Knpper, Katarzyna Jobin, Fabian Imdahl, Gosia Golda, Kathrin Hoh, Anika Grafen, Tsuneyasu Kaisho, Antoine-Emmanuel Saliba, Dominic Grün, Georg Gasteiger, Marc Bajénoff, Wolfgang Kastenmüller
Issue&Volume: 2023-07-17
Abstract: Unlike macrophage networks composed of long-lived tissue-resident cells within specific niches, conventional dendritic cells (cDCs) that generate a 3D network in lymph nodes (LNs) are short lived and continuously replaced by DC precursors (preDCs) from the bone marrow (BM). Here, we examined whether specific anatomical niches exist within which preDCs differentiate toward immature cDCs. In situ photoconversion and Prtn3-based fate-tracking revealed that the LN medullary cords are preferential entry sites for preDCs, serving as specific differentiation niches. Repopulation and fate-tracking approaches demonstrated that the cDC1 network unfolded from the medulla along the vascular tree toward the paracortex. During inflammation, collective maturation and migration of resident cDC1s to the paracortex created discontinuity in the medullary cDC1 network and temporarily impaired responsiveness. The decrease in local cDC1 density resulted in higher Flt3L availability in the medullary niche, which accelerated cDC1 development to restore the network. Thus, the spatiotemporal development of the cDC1 network is locally regulated in dedicated LN niches via sensing of cDC1 densities.
DOI: 10.1016/j.immuni.2023.06.020
Source: https://www.cell.com/immunity/fulltext/S1074-7613(23)00277-7
Immunity：《免疫》，创刊于1994年。隶属于细胞出版社，最新IF：43.474
官方网址：https://www.cell.com/immunity/home
投稿链接：https://www.editorialmanager.com/immunity/default.aspx
本期文章：《免疫》：Online/在线发表
