小柯机器人

美国波士顿大学Hadi T. Nia课题组实现在细胞分辨率探测实时肺功能的平台-晶体胸腔。2023年9月14日出版的《自然-方法学》发表了这项成果。

研究人员研发了晶体胸腔：一种透明的胸腔，其能在全器官以及单细胞水平对功能性肺进行多尺度光学成像。它能够通过血管内、肺内、实质内和光遗传学干预来调节肺部生物物理学和免疫，并保留了肺的三维结构、气液界面、细胞多样性和呼吸循环功能。将这些功能运用到存在肺部疾病的小鼠模型中，研究人员实现了对疾病进展期间单肺泡和毛细血管水平呼吸循环功能的重塑。

该晶体胸腔及其广泛应用将有助于对肺部疾病的进一步研究，并有助于发现新的治疗靶点。

据介绍，了解肺部疾病的动态发病机制和对治疗的反应需要在细胞分辨率实时探测肺部动态。尽管活体成像技术取得了进步，但在主动呼吸和循环过程中对肺部进行光学成像仍然具有挑战性。

附：英文原文

Title: Crystal ribcage: a platform for probing real-time lung function at cellular resolution

Author: Banerji, Rohin, Grifno, Gabrielle N., Shi, Linzheng, Smolen, Dylan, LeBourdais, Rob, Muhvich, Johnathan, Eberman, Cate, Hiller, Bradley E., Lee, Jisu, Regan, Kathryn, Zheng, Siyi, Zhang, Sue, Jiang, John, Raslan, Ahmed A., Breda, Julia C., Pihl, Riley, Traber, Katrina, Mazzilli, Sarah, Ligresti, Giovanni, Mizgerd, Joseph P., Suki, Bla, Nia, Hadi T.

Issue&Volume: 2023-09-14

Abstract: Understanding the dynamic pathogenesis and treatment response in pulmonary diseases requires probing the lung at cellular resolution in real time. Despite advances in intravital imaging, optical imaging of the lung during active respiration and circulation has remained challenging. Here, we introduce the crystal ribcage: a transparent ribcage that allows multiscale optical imaging of the functioning lung from whole-organ to single-cell level. It enables the modulation of lung biophysics and immunity through intravascular, intrapulmonary, intraparenchymal and optogenetic interventions, and it preserves the three-dimensional architecture, air–liquid interface, cellular diversity and respiratory–circulatory functions of the lung. Utilizing these capabilities on murine models of pulmonary pathologies we probed remodeling of respiratory–circulatory functions at the single-alveolus and capillary levels during disease progression. The crystal ribcage and its broad applications presented here will facilitate further studies of nearly any pulmonary disease as well as lead to the identification of new targets for treatment strategies. A fabricated transparent ribcage facilitates real-time analysis of whole-lung function down to cellular resolution.

DOI: 10.1038/s41592-023-02004-9

Source: https://www.nature.com/articles/s41592-023-02004-9

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex