小柯机器人

荷兰乌得勒支大学Lukas C. Kapitein团队开发出膨胀显微镜的本征校准和变形绘图的新方法。2023年9月18日，《自然—方法学》杂志在线发表了这项成果。

研究人员报道了GelMap，这是一种灵活的工作流程，可将荧光网格引入预膨胀水凝胶，使其随膨胀而缩放并报告变形情况。研究人员证明了GelMap可用于精确确定局部膨胀因子并校正变形，而无需使用细胞参考结构或膨胀前的实际图像。此外，研究人员还展示了GelMap对相关使用膨胀显微镜的样本导航的帮助。最后，研究人员还展示了GelMap与组织膨胀兼容，可作为质量控制步骤轻松应用到现有的膨胀显微镜（ExM）工作流程中。

据介绍，ExM是一种功能强大的技术，它通过在三维空间中物理膨胀生物样本来克服光显微镜的衍射极限。然而，将膨胀显微镜用于定量或诊断应用需要强大的质量控制方法，以精确确定膨胀因子并绘制各向异性膨胀导致的变形图。

附：英文原文

Title: GelMap: intrinsic calibration and deformation mapping for expansion microscopy

Author: Damstra, Hugo G. J., Passmore, Josiah B., Serweta, Albert K., Koutlas, Ioannis, Burute, Mithila, Meye, Frank J., Akhmanova, Anna, Kapitein, Lukas C.

Issue&Volume: 2023-09-18

Abstract: Expansion microscopy (ExM) is a powerful technique to overcome the diffraction limit of light microscopy by physically expanding biological specimen in three dimensions. Nonetheless, using ExM for quantitative or diagnostic applications requires robust quality control methods to precisely determine expansion factors and to map deformations due to anisotropic expansion. Here we present GelMap, a flexible workflow to introduce a fluorescent grid into pre-expanded hydrogels that scales with expansion and reports deformations. We demonstrate that GelMap can be used to precisely determine the local expansion factor and to correct for deformations without the use of cellular reference structures or pre-expansion ground-truth images. Moreover, we show that GelMap aids sample navigation for correlative uses of expansion microscopy. Finally, we show that GelMap is compatible with expansion of tissue and can be readily implemented as a quality control step into existing ExM workflows.

DOI: 10.1038/s41592-023-02001-y

Source: https://www.nature.com/articles/s41592-023-02001-y

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex