小柯机器人

上海交通大学医学院附属瑞金医院瞿介明等研究人员合作完成接种不同剂量灭活病毒疫苗的Omicron感染者的全身免疫谱分析。2023年9月27日，《细胞》杂志在线发表了这项成果。

在2022年上海爆发Omicron疫情期间，研究人员招募了122名感染成人和50名未感染对照者，他们未接种或接种过两剂或三剂COVID-19非活性疫苗，并对他们的外周血样本进行了41-plex CyTOF、RNA-seq和Olink的综合分析。结果显示，HLA-DR^hi经典单核细胞、非经典单核细胞和Th1样Tem的频率呈上升趋势，而Treg的频率则因加强疫苗而降低，它们以疫苗剂量依赖性的方式影响症状的发生。

相互关联和机理分析表明，加强免疫诱导了单核细胞训练，这将促进单核细胞的活化和成熟，而不是在Omicron感染后分化成髓源性抑制细胞。总之，这项研究为了解加强免疫是如何产生跨SARS-CoV-2变种的保护性免疫力提供了见解。

据介绍，以SARS-CoV-2原始毒株为基础的疫苗接种对Omicron变体引发的感染、症状发生和疾病严重程度具有保护作用，其方式与加强剂有关。然而，其潜在机制仍不清楚。

附：英文原文

Title: Systemic immune profiling of Omicron-infected subjects inoculated with different doses of inactivated virus vaccine

Author: Shanhe Yu, Yingni Lin, Yong Li, Shijun Chen, Lina Zhou, Hejie Song, Cuiping Yang, Haiqing Zhang, Jianping Zhou, Shunchang Sun, Yanan Li, Juan Chen, Ruixue Feng, Niu Qiao, Yinyin Xie, Ruihong Zhang, Tong Yin, Saijuan Chen, Qingyun Li, Jiang Zhu, Jieming Qu

Issue&Volume: 2023-09-27

Abstract: SARS-CoV-2 primary strain-based vaccination exerts a protective effect against Omicron variants-initiated infection, symptom occurrence, and disease severity in a booster-dependent manner. Yet, the underlying mechanisms remain unclear. During the 2022 Omicron outbreak in Shanghai, we enrolled 122 infected adults and 50 uninfected controls who had been unvaccinated or vaccinated with two or three doses of COVID-19 inactive vaccines and performed integrative analysis of 41-plex CyTOF, RNA-seq, and Olink on their peripheral blood samples. The frequencies of HLA-DRhi classical monocytes, non-classical monocytes, and Th1-like Tem tended to increase, whereas the frequency of Treg was reduced by booster vaccine, and they influenced symptom occurrence in a vaccine dose-dependent manner. Intercorrelation and mechanistic analysis suggested that the booster vaccination induced monocytic training, which would prime monocytic activation and maturation rather than differentiating into myeloid-derived suppressive cells upon Omicron infections. Overall, our study provides insights into how booster vaccination elaborates protective immunity across SARS-CoV-2 variants.

DOI: 10.1016/j.cell.2023.08.033

Source: https://www.cell.com/cell/fulltext/S0092-8674(23)00964-9