小柯机器人

以色列理工学院Amit Zeisel团队的最新研究揭示了小鼠杏仁核神经元类型及其对恐惧条件反射的转录反应。相关论文于2023年10月26日发表于国际学术期刊《自然—神经科学》杂志上。

研究人员揭示了成年小鼠杏仁核在恐惧学习和记忆巩固过程中的详细细胞类型分类。研究对野生小鼠和恐惧调节小鼠进行了单细胞RNA测序，鉴定了130种不同类型的神经元细胞，并验证了它们的空间分布。这些神经元类型中的一个子集对恐惧学习和记忆恢复具有转录反应。被激活的刻画细胞会上调活动反应基因，并协调与神经元突起、突触信号、可塑性和发育相关的基因的表达。

研究发现了已知和以前未知的对恐惧学习有反应的候选基因。该分子图谱可用于提出一些假说，以揭示调节学习和与情感有关记忆神经元的类型和神经回路。

据悉，杏仁核是主要参与情绪反应的脑区。利用遗传标记和单细胞转录组学可以深入了解与行为相关的细胞状态变化。

附：英文原文

Title: Neuronal types in the mouse amygdala and their transcriptional response to fear conditioning

Author: Hochgerner, Hannah, Singh, Shelly, Tibi, Muhammad, Lin, Zhige, Skarbianskis, Niv, Admati, Inbal, Ophir, Osnat, Reinhardt, Nuphar, Netser, Shai, Wagner, Shlomo, Zeisel, Amit

Issue&Volume: 2023-10-26

Abstract: The amygdala is a brain region primarily associated with emotional response. The use of genetic markers and single-cell transcriptomics can provide insights into behavior-associated cell state changes. Here we present a detailed cell-type taxonomy of the adult mouse amygdala during fear learning and memory consolidation. We perform single-cell RNA sequencing on nave and fear-conditioned mice, identify 130 neuronal cell types and validate their spatial distributions. A subset of all neuronal types is transcriptionally responsive to fear learning and memory retrieval. The activated engram cells upregulate activity-response genes and coordinate the expression of genes associated with neurite outgrowth, synaptic signaling, plasticity and development. We identify known and previously undescribed candidate genes responsive to fear learning. Our molecular atlas may be used to generate hypotheses to unveil the neuron types and neural circuits regulating the emotional component of learning and memory.

DOI: 10.1038/s41593-023-01469-3

Source: https://www.nature.com/articles/s41593-023-01469-3

Nature Neuroscience：《自然—神经科学》，创刊于1998年。隶属于施普林格·自然出版集团，最新IF：28.771
官方网址：https://www.nature.com/neuro/
投稿链接：https://mts-nn.nature.com/cgi-bin/main.plex