小柯机器人

美国加州大学洛杉矶分校Heather R. Christofk等研究人员合作中晚期妊娠和糖尿病妊娠的胎儿代谢图谱。相关论文于2023年12月8日在线发表在《细胞》杂志上。

研究人员展示了正常妊娠和糖尿病妊娠中晚期胎鼠体内代谢的描述性图谱。研究人员使用¹³C-葡萄糖和液相色谱-质谱（LC-MS）分析了胚胎10.5天到18.5天期间从怀孕母鼠身上采集的胎儿大脑、心脏、肝脏和胎盘的代谢情况。这些分析揭示了高血糖环境下特有的代谢特征，以及可能表示优血糖发育过程中发育转变的特征。研究人员观察到山梨醇在胎儿组织中的积累，以及从高血糖母体分离的胎儿大脑中神经递质水平的改变。

¹³C-葡萄糖追踪显示，胎儿的营养来源因母体血糖状态而异。无论血糖状态如何，组氨酸衍生代谢物都会在晚期胎儿组织中积累。这个丰富的数据集全面展示了体内胎儿组织代谢以及母体高血糖引起的变化。

据悉，越来越多的证据表明，新陈代谢会影响干细胞的命运。然而，胎儿的新陈代谢在发育过程中如何变化，以及母体新陈代谢的改变如何影响胎儿的新陈代谢，这些问题仍有待探索。

附：英文原文

Title: Atlas of fetal metabolism during mid-to-late gestation and diabetic pregnancy

Author: Cesar A. Perez-Ramirez, Haruko Nakano, Richard C. Law, Nedas Matulionis, Jennifer Thompson, Andrew Pfeiffer, Junyoung O. Park, Atsushi Nakano, Heather R. Christofk

Issue&Volume: 2023-12-08

Abstract: Mounting evidence suggests metabolism instructs stem cell fate decisions. However, how fetal metabolism changes during development and how altered maternal metabolism shapes fetal metabolism remain unexplored. We present a descriptive atlas of in vivo fetal murine metabolism during mid-to-late gestation in normal and diabetic pregnancy. Using 13C-glucose and liquid chromatography-mass spectrometry (LC-MS), we profiled the metabolism of fetal brains, hearts, livers, and placentas harvested from pregnant dams between embryonic days (E)10.5 and 18.5. Our analysis revealed metabolic features specific to a hyperglycemic environment and signatures that may denote developmental transitions during euglycemic development. We observed sorbitol accumulation in fetal tissues and altered neurotransmitter levels in fetal brains isolated from hyperglycemic dams. Tracing 13C-glucose revealed disparate fetal nutrient sourcing depending on maternal glycemic states. Regardless of glycemic state, histidine-derived metabolites accumulated in late-stage fetal tissues. Our rich dataset presents a comprehensive overview of in vivo fetal tissue metabolism and alterations due to maternal hyperglycemia.

DOI: 10.1016/j.cell.2023.11.011

Source: https://www.cell.com/cell/fulltext/S0092-8674(23)01228-X