小柯机器人

韩国科学技术院Won Do Heo等研究人员合作实现活细胞体内突触结构动态实时可视化。相关论文于2024年1月8日在线发表在《自然—方法学》杂志上。

研究人员表示，突触结构的可塑性对调节大脑功能至关重要。然而，由于分割荧光蛋白（FP）的不可逆结合，目前可用的基于分割FP的突触组织研究方法在评估体内突触动态方面受到了限制。

研究人员开发了“SynapShot”，这是一种通过将二聚化依赖性荧光蛋白（ddFP）与工程突触粘附分子相结合来观察完整突触结构动态的方法。SynapShot可实时监测生理刺激下突触触点的可逆双向变化。在SynapShot中应用绿色和红色ddFP可同时观察到两个不同的突触群。

值得注意的是，红移SynapShot与基于蓝光的光遗传学技术高度兼容，可在精确控制特定信号通路的同时实现突触动态的可视化。此外，研究人员还证明了SynapShot能够在原始行为和高阶行为中实时监测小鼠大脑中突触接触的结构变化。

附：英文原文

Title: Real-time visualization of structural dynamics of synapses in live cells in vivo

Author: Son, Seungkyu, Nagahama, Kenichiro, Lee, Jinsu, Jung, Kanghoon, Kwak, Chuljung, Kim, Jihoon, Noh, Young Woo, Kim, Eunjoon, Lee, Sangkyu, Kwon, Hyung-Bae, Heo, Won Do

Issue&Volume: 2024-01-08

Abstract: The structural plasticity of synapses is crucial for regulating brain functions. However, currently available methods for studying synapse organization based on split fluorescent proteins (FPs) have been limited in assessing synaptic dynamics in vivo due to the irreversible binding of split FPs. Here, we develop ‘SynapShot’, a method for visualizing the structural dynamics of intact synapses by combining dimerization-dependent FPs (ddFPs) with engineered synaptic adhesion molecules. SynapShot allows real-time monitoring of reversible and bidirectional changes of synaptic contacts under physiological stimulation. The application of green and red ddFPs in SynapShot enables simultaneous visualization of two distinct populations of synapses. Notably, the red-shifted SynapShot is highly compatible with blue light-based optogenetic techniques, allowing for visualization of synaptic dynamics while precisely controlling specific signaling pathways. Furthermore, we demonstrate that SynapShot enables real-time monitoring of structural changes in synaptic contacts in the mouse brain during both primitive and higher-order behaviors.

DOI: 10.1038/s41592-023-02122-4

Source: https://www.nature.com/articles/s41592-023-02122-4

Nature Methods：《自然—方法学》，创刊于2004年。隶属于施普林格·自然出版集团，最新IF：47.99
官方网址：https://www.nature.com/nmeth/
投稿链接：https://mts-nmeth.nature.com/cgi-bin/main.plex