小柯机器人

瑞士日内瓦大学Paul Guichard和Virginie Hamel课题组取得一项新突破。他们报道了人体中心粒组装分子结构的时间序列重建。相关论文于2024年4月10日发表在《细胞》杂志上。

为了揭示这一过程，该课题组研究人员使用膨胀显微技术，将24个中心粒蛋白的空间位置与结构特征联系起来。人类中心粒前组装过程中蛋白质分布的时间序列重建，揭示了中心粒生物发生步骤的分子结构。研究人员发现这个过程始于一个没有微管的裸侧轮的形成。接下来，随着微管叶片的装配，伴随着径向分离和快速的侧轮生长，生长阶段继续进行。

在随后的延伸阶段，微管蛋白骨架随着A-C连接体的募集而线性增长，随后是内侧支架(IS)蛋白的募集。通过以下六个结构模块，研究团队模拟了人体中心粒的4D组装。总的来说，这项工作为研究大分子的空间和时间组装提供了一个框架。

据介绍，中心粒的生物发生，如同在大多数细胞器聚集一样，涉及支持其功能的亚结构元件的连续募集。

附：英文原文

Title: Time-series reconstruction of the molecular architecture of human centriole assembly

Author: Marine H. Laporte, Davide Gambarotto, élose Bertiaux, Lorène Bournonville, Vincent Louvel, José M. Nunes, Susanne Borgers, Virginie Hamel, Paul Guichard

Issue&Volume: 2024-04-10

Abstract: Centriole biogenesis, as in most organelle assemblies, involves the sequential recruitment of sub-structural elements that will support its function. To uncover this process, we correlated the spatial location of 24 centriolar proteins with structural features using expansion microscopy. A time-series reconstruction of protein distributions throughout human procentriole assembly unveiled the molecular architecture of the centriole biogenesis steps. We found that the process initiates with the formation of a naked cartwheel devoid of microtubules. Next, the bloom phase progresses with microtubule blade assembly, concomitantly with radial separation and rapid cartwheel growth. In the subsequent elongation phase, the tubulin backbone grows linearly with the recruitment of the A-C linker, followed by proteins of the inner scaffold (IS). By following six structural modules, we modeled 4D assembly of the human centriole. Collectively, this work provides a framework to investigate the spatial and temporal assembly of large macromolecules.

DOI: 10.1016/j.cell.2024.03.025

Source: https://www.cell.com/cell/fulltext/S0092-8674(24)00316-7