背景回顾：Genetic gain in potato is hampered by the heterozygous tetraploid genome of cultivated potato. Converting potato into a diploid inbred-line based F1-hybrid crop provides a promising route towards increased genetic gain. The introduction of a dominant S-locus inhibitor (Sli) gene into diploid potato germplasm allows efficient generation of self-fertilized seeds and thus the development of potato inbred lines. 

提出问题：Little is known about the structure and function of the Sli locus. 

结果1-QTL定位：Here we describe the mapping of Sli to a 12.6 kb interval on chromosome 12 using a recombinant screen approach. 

结果2-候选基因：One of two candidate genes present in this interval shows a unique sequence that is exclusively present in self-compatible lines. 

结果3-实验验证：We describe an expression vectorthat converts self-incompatible genotypes into self-compatible and a CRISPR-Cas9 vector that converts SC genotypes into SI. 

结果4-关键基因：The Sli gene encodes an F-box protein that is specifically expressed in pollen from self-compatible plants. 

结果5-关键突变：A 533 bp insertion in the promotor of that gene leads to a gain of function mutation, which overcomes self-pollen rejection.

 摘 要 

马铃薯育种改良的遗传增益受到栽培种马铃薯四倍体杂合基因组的限制。基于F1代杂种马铃薯，将其转变为二倍体自交系，将是提高马铃薯遗传增益的一条潜力途径。将一个显性S位点抑制因子Sli基因引入马铃薯二倍体种质，可以有效地产生自交种子，从而发展马铃薯自交系。但是，我们对于Sli基因座的结构和功能知之甚少。本文中，作者通过一种重组扫描的方法，将Sli基因座map到了马铃薯12号染色体上一段12上kb的序列上。该区间上存在两个候选基因，其中一个包含一段特异性的序列，该序列只存在于自交亲和的株系中。作者构建了一个将自交不亲和基因型转化为自交亲和基因型的表达载体，和一个将自交亲和基因型转化为自交不亲和基因型的CRISPR-Cas9载体。Sli基因编码一个F-box蛋白，在自交亲和株系的花粉中特异性表达。一段533 bp的序列插入到该基因的启动子中，导致了功能获得性突变，从而克服了对于自身花粉的排斥。