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REHAB Project Sampling Analysis Plan
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Sampling Analysis Plan (SAP) was developed by King County for the tri-county Regional Examination of Harmful Algal Blooms (REHAB) project. It lays out the project design, sampling methods and laboratory analysis methods.
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研究藻类水华方面的，可以去储存室下载这个文件CDC REHAB 2009_SAP_Final.pdf。
SAMPLING AND ANALYSIS PLAN FOR THE REGIONAL EXAMINATION OF HARMFUL ALGAL BLOOMS (REHAB)
TABLE OF CONTENTS
1 INTRODUCTION
1.1 PROJECT BACKGROUND
1.2 REGULATORY STATUS OF CYANOTOXIN CRITERIA AND GUIDELINES
1.3 STUDY AREA DESCRIPTION
1.4 PROJECT OBJECTIVES
2 STUDY DESIGN
2.1 APPROACH
2.2 TIMELINE
2.3 SAMPLING PROCEDURES
2.3.1 Water sample collection and storage procedure to test for toxins:
2.3.2 Water sample collection and storage procedure for quantitative identification of cyanobacteria.
3 LABORATORY ANALYSIS
3.1 SAMPLE PREPARATION FOR ALGAL TOXIN ELISA
3.1.1 Cell Lysing Procedure for Microcystins and Cylindrospermopsin
1 INTRODUCTION
1.1 PROJECT BACKGROUND
1.2 REGULATORY STATUS OF CYANOTOXIN CRITERIA AND GUIDELINES
1.3 STUDY AREA DESCRIPTION
1.4 PROJECT OBJECTIVES
2 STUDY DESIGN
2.1 APPROACH
2.2 TIMELINE
2.3 SAMPLING PROCEDURES
2.3.1 Water sample collection and storage procedure to test for toxins:
2.3.2 Water sample collection and storage procedure for quantitative identification of cyanobacteria.
3 LABORATORY ANALYSIS
3.1 SAMPLE PREPARATION FOR ALGAL TOXIN ELISA
3.1.1 Cell Lysing Procedure for Microcystins and Cylindrospermopsin
3.1.2 Sample Preparation: Preservation Procedure for Saxitoxin
3.1.3 Microcystins– ELISA
3.1.4 Cylindrospermopsin ELISA
3.1.5 Saxitoxin ELISA
3.2 ANATOXIN-A
3.2.2. Chromatography3.2.3 Method Detection Limits
3.2 ANATOXIN-A
3.2.2. Chromatography3.2.3 Method Detection Limits
3.3 ANALYTICAL PROCEDURES
3.4 LABORATORY MICROSCOPIC ANALYSES OF PHYTOPLANKTON SAMPLES
4 DATA QUALITY OBJECTIVES
4.1 LABORATORY PRECISION4.2 FIELD PRECISION
4.3 BIAS
4.4 REPRESENTATIVENESS
4.4.1 Representativeness and precision of phytoplankton density estimates
4.6 COMPLETENESS
5 DATA REDUCTION, REVIEW, AND REPORTING
5.1 TOXIN DATA.
4.3 BIAS
4.4 REPRESENTATIVENESS
4.4.1 Representativeness and precision of phytoplankton density estimates
4.6 COMPLETENESS
5 DATA REDUCTION, REVIEW, AND REPORTING
5.1 TOXIN DATA.
6 PROJECT ORGANIZATION.
7 QUALITY CONTROL PROCEDURES
7.1 FIELD QUALITY CONTROL PROCEDURES
7.2 LABORATORY QUALITY CONTROL PROCEDURES
7.2.1 Frequency of quality control samples
7.3 CORRECTIVE ACTION
8 REFERENCES
7 QUALITY CONTROL PROCEDURES
7.1 FIELD QUALITY CONTROL PROCEDURES
7.2 LABORATORY QUALITY CONTROL PROCEDURES
7.2.1 Frequency of quality control samples
7.3 CORRECTIVE ACTION
8 REFERENCES
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