通过基因ID文件从fasta文件中提取特定的基因序列 Extract sequence with header from a fasta file with specific ID given in another file 最近在进行一些转录组数据的挖掘和分析实验,在实际的数据处理中,往往需要对目标序列文件进行基于一定条件的筛选,比如本文中所提到的通过基因ID文件从fasta文件中提取得到 ...
Recently, I need some RNA-seq experiments which have been published in NCBI for my study, while large sra file would take a lot of time in raw-data downloading (i.e. wget downloading). So I want to use aspera connect (command line version) to increse the rate for file downloading. The detail descr ...
最近在Ubuntu server中安装Blast2Go时出现报错: Blast2GOCommand Line V1.0.2 build: 31032014 It seems that you ara executing Blast2GO with aversion of OpenJDK Java. Please use themost recent version from Oracle. 表示需要最新版本的OpenJDK Java。经过几番折腾,终于将最新版本的OpenJ ...
FPKM, Fragments Kilobase of exon model per millon mapped reads, which can be used to indicate the expression (abundance) characteristics of genes. Now I will describe operation about obtaining interested gene FPKM value. 1.Software Download 1).fastq-dump: convert sra file to fastq file. ...
GEOquery is a tool which can be use to obtain GEO database from NCBI. Today I tried to install this packages in R, but I met some questions. my operation as follows: source('http://bioconductor.org/biocLite.R') Bioconductor version 2.14 (BiocInstaller 1.14.2), ?biocLite for help biocLit ...